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Proceedings of the International Plant Propagator's Society

Vol. 46

Effects of Media and Time of Seed Collection on Seed Germination of Cypripedium macranthum var. rebunense

M. Tomita

pp: 730-734

To obtain basic information for maximizing the in vitro seed germination of Cypripedium macranthum var. rebunense the suitability of media and the optimum time of seed collection were investigated. Seed capsules were collected at weekly intervals ranging from 5 to 8 weeks after pollination and inoculated on four tested media (Harvais, ½ MS, ½ Norstog, and T). Seeds collected at 6 weeks after pollination had the highest germination regardless of the medium components. Germinations and subsequent growth on both °Norstog and T medium were better than those on Harvais and ° MS medium. Eighty weeks after inoculation, seedlings were transplanted to soil-based media, and a preliminary investigation was made of the relation of cold treatment to sprouting (shoot elongation).

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