Plant Tissue Culture, Dispelling the Mystique
Brian J. Callaghan
I have been involved in commercial propagation of plants using tissue culture techniques since 1981. Over these last 15 years, I have seen a dramatic change in our nursery customers' attitudes towards the use of tissue-cultured plants. The need to produce plants in a cloistered environment of a laboratory decked out like an operating theatre, has made it difficult for the average propagator to relate to tissue culture in a similar way as she/he would when producing plants by traditional cuttings or by seed propagation. Nowadays these techniques are used as an everyday tool to bulk up certain lines. In this paper I will discuss the simple steps in the tissue culture process and try to dispel some of the associated myths.
I intend to use the main crop produced at Lifetech Laboratories as an example of how tissue culture can be applied to commercialise this important crop.
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