In Vitro Culture and Micrografting of White Pine Meristems
Barry Goldfarb, Gail E. McGill, Wesley P. Hackett and Olivier Monteuuis
Our long-term research goal is to develop a vegetative propagation method for mature eastern white pine (Pinus strobus L.) trees. Unfortunately, rooting from cuttings of white pines, like most other conifers, decreases with increasing plant age. Rejuvenation, that is restoration of rootability to mature tissue, may be possible by tissue culturing or grafting shoot apical meristems. Recently, rejuvenated shoots have been obtained via meristem culture from Sierra redwood, Sequoiadendron giganteum, (Monteuuis, 1991) and via meristem micrografting from maritime pine, Pinus pinaster, (Dumas et al., 1989) If rejuvenated shoots can be obtained from mature white pines, they will be used as a stock block source of rootable cuttings to clonally propagate superior genotypes. In this paper we describe our efforts to develop methods for tissue culturing and micrografting of white pine meristems.
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