Vol. 30

Title:
COMMERCIALLY-FEASIBLE MICROPROPAGATION OF MOUNTAIN LAUREL, KALMIA LATIFOLIA, BY USE OF SHOOT-TIP CULTURE

Authors:
Gregory Lloyd and Brent McCown

pp: 421-426

Abstract:
The multiplication at rates feasible for commercial production of mountain laurel, Kalmia latifolia, by micropropagation using shoot-tip cultures has been demonstrated. Shoot-tip explants placed initially in liquid woody plant medium (WPM) supplemented with 4–16 µM N⁶-(Δ₂-isopentenyl)-adenine (2iP) produced axillary shoots by 1 to 2 months. These new shoots were excised from the original explant and placed on the same WPM solidified with agar. The resultant shoot mass was subcultured monthly. Actively multiplying shoot-tip cultures were produced within 6 months. A comparison of 7 concentrations of 2iP, varying from 0 to 64 µM, showed that a concentration of 8 µM 2iP produced the greatest number of utilizable shoots after 8 weeks in culture. Stock cultures were maintained or increased monthly by removing and subculturing shoots elongating from the basal mass. Thirty to forty utilizable shoots were harvested from each culture 6 to 8 weeks after the initial subculture. Multiplication rates of 8 to 10 times were readily achieved. Harvested shoots rooted with 73% success in 4 to 6 weeks when placed in a 100% peat medium in a high humidity chamber. After a period acclimation, these plants can be treated like young seedlings in commercial production.

Full text: IPPS members     ISHS members & pay-per-view
(PDF 1546597 bytes)

Translate:

           

IPPS membership administration       ISHS membership administration