VIRUS ELIMINATION AND RAPID PROPAGATION OF GRAPES IN VITRO
Robert E. Harris and John H. Stevenson
Heat treatment and in vitro culture of shoot tips were used to free Vitis vinifera ‘Liemberger’ of leafroll virus and ‘Forta’ and ‘Auxerrois’ (‘Cl-21’) of fanleaf virus. Rapid propagation of the French hybrid ‘Baco’ was obtained on full-strength MS medium plus adenine sulfate (80 mg/l), NaH2PO4 H2O (170 mg/l), i-inositol (100 mg/l), thiamine-HCl (0.4 mg/l), and BAP (3 to 4 mg/l). Rooting of proliferated shoots was most rapid on ¼-strength MS with 0.08 mg/l IBA. The method appears suitable for the rapid propagation of other cultivars.
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