Eur.J.Hortic.Sci. 82 (1) 54-60 | DOI: 10.17660/eJHS.2017/82.1.6|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2017 | European Journal of Horticultural Science | Original article
In vitro propagation of medicinal and ornamental plant Lysimachia davurica
Yanni Zhang, Dongling Sun and Shangchun Hu
College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China
Lysimachia davurica is an important medicinal and ornamental plant species. An efficient in vitro regeneration system on excised leaves of L. davurica was developed. The experimental conditions of tissue culture system were investigated based on the effect of different concentrations and combinations of plant growth regulators (PGRs), explant orientations, leaf segments, and concentrations of activated charcoal. Two types of callus induction medium using Murashige and Skoog (MS) medium + 1.0 mg L-1 NAA + 0.5 mg L-1 6-BA and 1.0 mg L-1 NAA + 1.0 mg L-1 6-BA had a callus inducing rate of 95.56%. The shortest time to callus appearance was 7 d. Proximal segments were better for callus induction than middle and distal ones. Placing leaf with abaxial side down on medium was better than adaxial side for inoculation. For the combinations of 6-BA and NAA tested, MS + 0.5 mg L-1 6-BA and 0.1 mg L-1 NAA was best for callus proliferation, while MS + 1.0 mg L-1 6-BA and 0.1 mg L-1 NAA was optimal for callus differentiation and shooting. For the combinations of PGRs tested, the best rooting medium is 1/2 MS + 0.5 mg L-1 IBA + 0 mg L-1 NAA, with 99.89% rooting rate. On plantlets rooting, 1 g L-1 activated charcoal performed better than others (0, 2, 3, and 4 g L-1). The optimal transplanting medium was using vermiculite and humus as substrate in the proportion of 2:1 (v/v), with 97.78% survival rate. This protocol could be an efficient method for the micropropagation of L. davurica.
micropropagation, tissue culture, leaf, shoot regeneration, callus
Significance of this study
What is already known on this subject?
What are the new findings?
Micropropagation of L. davurica had been achieved using hypocotyl and cotyledon stem section as explants while its efficiency of plant regeneration is relatively low. To date, a tissue culture protocol for regeneration of L. davurica using leaf tissue explants has not been reported.
What is the expected impact on horticulture?
Our study provides a tissue culture protocol for regeneration of L. davurica using leaf tissue explants, based on the effect of different hormone concentrations and combinations, explant orientations, leaf segments, and concentrations of activated charcoal.
The results could help promote rapid production of L. davurica for growers. The in vitro plantlets may be suitable to set up a transformation system to facilitate the production of transgenic plants with desirable horticultural traits or medicinal contents.
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Received: 10 October 2016 | Accepted: 12 January 2017 | Published: 23 February 2017 | Available online: 23 February 2017