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  Eur.J.Hortic.Sci. 82 (1) 31-37 | DOI: 10.17660/eJHS.2017/82.1.4
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2017 | European Journal of Horticultural Science | Original article

Water withholding contributes to winter hardiness in perennial ryegrass (Lolium perenne L.)

Zhihui Chang1, Bihui Sun1 and Deying Li2
1Forestry College, Beijing Forestry University, Beijing 100083, China
2Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA

SUMMARY
Perennial ryegrass (Lolium perenne L.) is an important turf and forage species in mild temperate regions. In cold regions, it is vulnerable to winter kill. The objective of this study was to investigate possible associations between cold tolerance and morphological traits that regulate water movement in perennial ryegrass. Cultivars ‘Uno’, ‘Brightstar SLT’, ‘Fiesta’, ‘Evening Shade’, ‘NK-200’, and ‘Linn’, representing different levels of cold tolerance and winter hardiness, were used in the study. Previously reported cold tolerant cultivars, ‘Uno’ and ‘Brightstar SLT’, showed smaller but higher density of stomata than cold sensitive cultivars such as ‘Linn’ and ‘NK-200’. In addition, cold tolerant cultivars had either higher density of short cells or higher density of hairs in the epidermis compared to the cold sensitive cultivars. Water wetting angles on the upper epidermis also indicated that ‘Uno’ had a better protection from water loss than cold sensitive cultivars, especially ‘Linn’. The water loss rate (% per day) was significantly higher for ‘Linn’ than ‘Uno’, which were 3.39 and 2.27, respectively. Simulated wind desiccation combined with freezing at -10°C after cold acclimation further showed that stronger water withholding ability contributed to the increased freezing tolerance. The results suggest that including water conserving morphologies in the selection process may help breeding for freeze tolerant perennial ryegrass.

Keywords abiotic stress, cold tolerance, low temperature, transpiration, turfgrass, winter kill

Significance of this study

What is already known on this subject?

  • Winter kill in perennial ryegrass can be a result of a direct exposure to low temperature, desiccation, suffocation under ice and snow, and toxic gas.
What are the new findings?
  • Stomata size/density, hair density, and epicuticular wax are linked to water conservation, which in turn affected low temperature kill in perennial ryegrass.
What is the expected impact on horticulture?
  • Breeders may include those water conservation traits in future selection for winter hardiness in perennial ryegrass.

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E-mail: changzh@bjfu.edu.cn  deying.li@ndsu.edu  

References

  • Abeynayake, S.W., Byme, S., Nagy, I., Jonaviciene, K., Etzerodt, T.P., Boelt, B., and Asp, T. (2015). Changes in Lolium perenne transcriptome during cold acclimation in two genotypes adapted to different climatic conditions. BMC Plant Biology 15, 250. https://doi.org/10.1186/s12870-015-0643-x.

  • Aleliunas, A., Jonaviciene, K., Statkeviciute, G., Vaitiekunaite, D., Kemesyte, V., Lubberstedt, T., and Brazauskas, G. (2015). Association of single nucleotide polymorphisms in LpIRI1 gene with freezing tolerance traits in perennial ryegrass. Euphytica 204, 523–534. https://doi.org/10.1007/s10681-014-1330-y.

  • Bates, L.S., Waldren, R.P., and Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil 39, 205–207. https://doi.org/10.1007/BF00018060.

  • Beard, J.B. (1969). Winter injury of turfgrasses. Proc. Int. Turfgrass Res. Conf., 15-18 July 1969. (Harrogate, England), p. 226–234.

  • Bocian, A., Zwierzykowski, Z., Rapacz, M., Kozayk, G., Ciesiolka, D., and Kosmala, A. (2015). Metabolite profiling during cold acclimation of Lolium perenne genotypes distinct in the level of frost tolerance. J. Appl. Genetics 56, 439–449. https://doi.org/10.1007/s13353-015-0293-6.

  • Cohen, R.P., and Wood, G.M. (1986). Predicting cold tolerance in perennial ryegrass trough alcohol bath freezing of seedling plants. Agronomy J. 78, 560–563.

  • Dalmannsdottir, S., Rapacz, M., Jorgensen, M., Ostrem, L., Larsen, A., Rodven, R., and Rognli, O.A. (2016). Temperature before cold acclimation affects cold tolerance and photoacclimation in timothy (Phleum pretense L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pretense L.). J. Agron. Crop Sci. 202, 320–330. https://doi.org/10.1111/jac.12149.

  • Eagles, C.F. (1989). Temperature-induced changes in cold tolerance of Lolium perenne. J. Agric. Sci. (Cambridge) 113, 339–347. https://doi.org/10.1017/S0021859600070027.

  • Ebdon, J.S., Gagne, R.A., and Manley, R.C. (2002). Comparative cold tolerance in diverse turf quality genotypes of perennial ryegrass. HortScience 37, 826–830.

  • Fulier M.P., and Eagles, C.F. (1978). A seedling test for cold hardiness in Lolium perenne L. J. Agri. Sci. (Cambridge) 91, 217–222. https://doi.org/10.1017/S0021859600056781.

  • Funk, C.R., and Clarke, B.B. (1989) Turfgrass breeding – with special reference to turf-type perennial ryegrass, tall fescue and endophytes. In Proceedings of the 6th International Turfgrass Research Conference, 31 July - 5 August, Tokyo, Japan. p. 3–10.

  • Gao, Y., and Li, D. (2015). Assessing leaf senescence in tall fescue (Festuca arundinacea Schreb.) under salinity stress using leaf spectrum. Europ. J. Hort. Sci. 80, 170–176. https://doi.org/10.17660/eJHS.2015/80.4.4.

  • Gay, A.P., and Eagles, C.F. (1991). Quantitative analysis of cold hardening and dehardening in Lolium. Annals of Botany 67, 339–345.

  • Gusta, L.V., Butler, J.D., Rajashekar, C., and Burke, M.J. (1980). Freezing resistance of perennial turfgrasses. HortScience 15, 494–496.

  • Hisano, H., Kanazawa, A., Yoshida, M., Humphreys, M.O., Lizuka, M., Kitamura, K., and Yamada, T. (2008). Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass. New Phytologist 178, 766–780. https://doi.org/10.1111/j.1469-8137.2008.02409.x.

  • Hoffman, L., Dacosta, M., Ebdon, J.S., and Watkins, E. (2010). Physiological changes during cold acclimation of perennial ryegrass accessions differing in freeze tolerance. Crop Sci. 50, 1037–1047. https://doi.org/10.2135/cropsci2009.06.0293.

  • Hoffman, L., Dacosta, M., Ebdon, J.S., and Zhao, J. (2012). Effects of drought preconditioning on freezing tolerance of perennial ryegrass. Environmental Experi. Botany 79, 11–20. https://doi.org/10.1016/j.envexpbot.2012.01.002.

  • Hulke, B.S., Watkins, E., Wyse, D., and Ehlke, N. (2007). Winterhardiness and turf quality of accessions of perennial tyegrass (Lolium perenne L.) form public collections. Crop Sci. 47, 1596–1602. https://doi.org/10.2135/cropsci2006.10.0671.

  • Hulke, B.S., Watkins, E., Wyse, D., and Ehlke, N. (2008). Freezing tolerance of selected perennial ryegrass (Lolium perenne L.) accessions and its association with field winterhardiness and turf traits. Euphytica 163, 131–141. https://doi.org/10.1007/s10681-007-9631-z.

  • Hulke, B.S., Bushman, B.S., Watkins, E., and Ehlke, N.J. (2012). Association of freezing tolerance to LpCBFIIIb and LpCBFIIIc gene polymorphism in perennial ryegrass accessions. Crop Sci. 52, 2023–2029. https://doi.org/10.2135/cropsci2011.09.0527.

  • Humphreys, M.O., and Eagles, C.F. (1988). Assessment of perennial ryegrass (Lolium perenne L.) for breeding. I. Freezing tolerance. Euphytica 38, 75–84. https://doi.org/10.1007/BF00024813.

  • Humphreys, M.O. (1989). Assessment of perennial ryegrass (Lolium perenne L.) for breeding. II. Components of winter hardiness. Euphytica 41, 99–106. https://doi.org/10.1007/BF00022418.

  • Iraba, A., Castonguay, Y., Bertrand, A., Floyd, D.J., Cloutier, J., and Belzile, F. (2013). Characterization of populations of turf-type perennial ryegrass recurrently selected for superior freezing tolerance. Crop Sci. 53, 2225–2238. https://doi.org/10.2135/cropsci2013.02.0100.

  • Jorgensen, M., Østrem, L., and Hoglind, M. (2009). De-hardening in contrasting cultivars of timothy and perennial ryegrass during winter and spring. Grass and Forage Science 65, 38–48. https://doi.org/10.1111/j.1365-2494.2009.00718.x.

  • Jung, G.A., and Kocher, R.E. (1974). Influence of applied nitrogen and clipping treatments on winter survival of perennial cool-season grasses. Agronomy J. 66, 62–65. https://doi.org/10.2134/agronj1974.00021962006600010016x.

  • Lawrence, T., Cooper, J.P., and Breese, E.L. (1973). Cold tolerance and winter hardiness in Lolium perenne II. Influence of light and temperature during growth and hardening. J. Agric. Sci. 80, 341–348. https://doi.org/10.1017/S0021859600057828.

  • Lorenzetti, F., Tyler, B.F., Cooper, J.P., and Breese, E.L. (1971). Cold tolerance and winter hardiness in Lolium perenne 1. Development of screening techniques for cold tolerance and survey of geographical variation. J. Agric. Sci. (Cambridge) 76, 199–209. https://doi.org/10.1017/S0021859600025545.

  • Moing, A., Carbonne, F., Rashad, M.H., and Gaudillere, J.P. (1992). Carbonfluexes in mature peach leaves. Plant Physiol. 100, 1878–1884. https://doi.org/10.1104/pp.100.4.1878.

  • Ostrem, L., Rapacz, M., Jorgensen, M., and Hoglind, M. (2010). Impact of frost and plant age on compensatory growth in timothy and perennial ryegrass during winter. Grass and Forage Sci. 65, 15–22. https://doi.org/10.1111/j.1365-2494.2009.00715.x.

  • Paina, C., Byrne, S.L., Domnisoru, C., and Asp, T. (2014). Vernalization mediated changes in the Lolium perenne transcriptome. Plos One 9: e107365. https://doi.org/10.1371/journal.pone.0107365.

  • Sandve, S.R., Kosmala, A., Rudi, H., Fjellheim, S., Rapacz, M., Yamada, T., and Rognli, O.A. (2011). Molecular mechanisms underlying frost tolerance in perennial grasses adapted to cold climates. Plant Sci. 180, 69–77. https://doi.org/10.1016/j.plantsci.2010.07.011.

  • Sarkar, E., Bhowmik, P.C., Kwon, Y., and Shetty, K. (2009). Cold acclimation responses of three cool-season turfgrasses and the role of proline-associated pentose phosphate pathway. J. Amer. Soc. Hort. Sci. 134, 210–220.

  • Scott, T.A., and Melvin, E.H. (1953). Determination of dextran with anthrone. Anal. Chem. 25, 1656–1661. https://doi.org/10.1021/ac60083a023.

  • Tcacenco, F.A., Eagles, C.F., and Tyler, B.F. (1989). Evaluation of winter hardiness in Romanian introductions of Lolium perenne. J. Agric. Sci. (Cambridge) 112, 249–255. https://doi.org/10.1017/S0021859600085142.

  • Thomson, A.J. (1974). The examination of some winter hardiness components in grasses using canonical variate analysis. J. Agric. Sci. 83, 545–550. https://doi.org/10.1017/S0021859600027039.

  • Waldron, B.L., Ehlke, N.J., Vellekson, D.J., and White, D.B. (1998a). Controlled freezing as an indirect selection method for field winterhardiness in turf-type perennial ryegrass. Crop Sci. 38, 811–816. https://doi.org/10.2135/cropsci1998.0011183X003800030032x.

  • Waldron, B.L., Ehlke, N.J., Wyse, D.L., and Wellekson, D.J. (1998b). Genetic variation and predicted gain from selection for winterhardiness and turf quality in a perennial ryegrass topcross population. Crop Sci. 38, 817–822. https://doi.org/10.2135/cropsci1998.0011183X003800030033x.

  • Warnock, D.L., Leep, R.H., Bughrara, S.S., and Min, D. (2005). Cold tolerance evaluation of improved diploid and tetraploid cultivars of perennial ryegrass. Online. Crop Management. https://doi.org/10.1094/CM-2005-0221-01-RS.

  • Webster, D.E., and Ebdon, J.S. (2005). Effects of nitrogen and potassium fertilization on perennial ryegrass cold tolerance during deacclimation in late winter and early spring. HortScience 40, 842–849.

  • White, D.B., and Smithberg, M.H. (1980). Cold acclimation and deacclimation in cool-season grasses. In Proceedings of the Third International Turfgrass Research Conference. 11-13 July 1977, Munich, West Germany. p. 149–154.

  • Wolfe, J., and Bryant, G. (1999). Freezing, drying, and/or vitrification of membrane-solute-water systems. Cryobiology 39, 103–129. https://doi.org/10.1006/cryo.1999.2195.

  • Wood, G.M., and Cohen, R.P. (1983). Predicting cold tolerance in perennial ryegrass from subcrown internode length. Agron. J. 76, 516–517. https://doi.org/10.2134/agronj1984.00021962007600040003x.

  • Xiong, Y., and Fei, S. (2006). Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.). Planta 224, 878–888. https://doi.org/10.1007/s00425-006-0273-5.

  • Xiong, Y., Fei, S., Arora, R., Brummer, E.C., Barker, R.E., Jung, G., and Warnke, S.E. (2007). Identification of quantitative trait loci controlling winter hardiness in an annual × perennial ryegrass interspecific hybrid population. Mol. Breeding 19, 125–136. https://doi.org/10.1007/s11032-006-9050-1.

  • Yamada, T., Jones, E.S., Cogan, N.O.I., Vecchies, A.C., Nomura, T., Hisano, H., Shimamoto, Y., Smith, K.F., Hayward, M.D., and Forster, J.W. (2004). QTL analysis of morphological, developmental, and winter hardiness-associated traits in perennial ryegrass. Crop Sci. 44, 925–935. https://doi.org/10.2135/cropsci2004.9250.

  • Yu, X., Pijut, P.M., Byrne, S., As, T., Bai, G., and Jiang, Y. (2015). Candidate gene association mapping for winter survival and spring regrowth in perennial ryegrass. Plant Sci. 235, 37–45. https://doi.org/10.1016/j.plantsci.2015.03.003.

  • Zhang, C., Fei, S., Warnke, S., Li, L., and Hannapel, D. (2009). Identification of genes associated with cold acclimation in perennial ryegrass. J. Plant Physiology 166, 1436–1445. https://doi.org/10.1016/j.jplph.2009.03.001.

  • Zhang, C., Fei, S., Arora, R., and Hannapel, D. (2010). Ice recrystallization inhibition proteins of perennial ryegrass enhance freezing tolerance. Planta 232, 155–164. https://doi.org/10.1007/s00425-010-1163-4.

  • Zhao, H., and Bughrara, S.S. (2008). Isolation and characterization of cold-regulated transcriptional activator LpCBF3 gene from perennial ryegrass (Lolium perenne L.). Mol. Genet. Genomics 279, 585–594. https://doi.org/10.1007/s00438-008-0335-4.

Received: 11 November 2016 | Accepted: 11 January 2017 | Published: 23 February 2017 | Available online: 23 February 2017

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