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  Eur.J.Hortic.Sci. 81 (6) 310-320 | DOI: 10.17660/eJHS.2016/81.6.4
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article

The impact of partial dehydration on grape and wine chemical composition of white grapevine (Vitis vinifera L.) varieties

J. Reščič, M. Mikulič-Petkovšek and D. Rusjan
Chair for Fruit Growing, Viticulture and Vegetable Growing, Department of Agronomy, Biotechnical Faculty, University of Ljubljana, SI-1000 Ljubljana, Slovenia

The aim of this study was to investigate the impact of Double Maturation Raisonnée (DMR) partial dehydration technique on chemical composition of two white and high-yielding grapevine varieties: 'Rebula' and 'Vitovska grganja' in two consecutive years. DMR technique was applied, where a cut of one-year old cane two to three weeks before harvest was done and grape remained on the vine till suitable chemical composition was reached. Grape and wine characteristics were monitored along with primary metabolites and phenolic profiles of berry skin and wine. DMR decreased the weight of 100 berries and caused an increase in soluble solids, titratable acidity, individual sugars and organic acids in comparison with the control berries. The wines produced from DMR grapes had higher alcohol content and total extracts. An interesting impact of DMR was observed on phenolic compounds. Procyanidin dimer 1 averagely increased for 22.0 mg kg-1 in 'Rebula' DMR berry skin. Similarly, 69.5 mg kg-1 more quercetin-3-glucoside was determined in 'Vitovska grganja' DMR berry skin compared to the control. In our study, DMR influenced and modified differently but significantly the grape and wine composition, especially the phenolic profiles of two white varieties and contributed to higher wine quality. The results propose the implementation of DMR partial dehydration technique for high-yielding white grapevine varieties in order to produce richer, full-bodied wines.

Keywords canopy management, phenolic maturation, partial dehydration, quality, 'Rebula', 'Vitovska grganja'

Significance of this study

What is already known on this subject?

  • Vineyard canopy practices and dehydration techniques are one of the main tools to manage and improve grape and wine quality for the production of different wine styles.
What are the new findings?
  • This study clearly demonstrated that DMR partial dehydration in the vineyard increased soluble solids and sugars, alcohol content and most of individual phenolic compounds determined in local, white ‘Rebula’ and ‘Vitovska grganja’ (Vitis vinifera L.) grapes and wine.
What is the expected impact on horticulture?
  • The DMR partial dehydration technique could be of much importance for winegrowers and winemakers, who struggle with difficulties in production of desired wine styles, demanded by the consumers, especially dealing with high-yielding varieties and high production costs.

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  • Bauer, R., and Dicks, L. (2004). Control of malolactic fermentation in wine. A review. S. Afr. J. Enol. Vitic. 25, 74–88.

  • Bonghi, C., Rizzini, F.M., Gambuti, A., Moio, L., Chkaiban, L., and Tonutti, P. (2012). Phenol compound metabolism and gene expression in the skin of wine grape (Vitis vinifera L.) berries subjected to partial postharvest dehydration. Postharvest Biol. Technol. 67, 102–109.

  • Canals, R., Llaudy, M.C., Valls, J., Canals, J.M., and Zamora, F. (2005). Influence of ethanol concentration on the extraction of color and phenolic compounds from the skin and seeds of tempranillo grapes at different stages of ripening. J. Agric. Food Chem. 53, 4019–4025.

  • Carbonneau, A., and Murisier, F. (2009). Doublewithering on vine: an innovating technique for reasoning maturation useful for sustainable viticulture. An alternative to sugar adding. Synthesis of experiments. Bulletin de l’OIV. Office International de la Vigne et du Vin (OIV, Paris, France), p.17–31.

  • Cargnello, G. (2000). Researches on the influence of the manipulation of canopy, of production and of grapes ripening on instrumental and sensory quality, on "economic quality" and on "quality economy" of production, in "new" models of integrated viticulture. Acta Hortic. 526, 75–80.

  • Cargnello, G., Pezza, L., Gallo, G., Camatta, T., Coccato, S., Pascarella, G., Di Gaetano, R., Casadei, G., La Torre, A., and Spera, G. (2005). DMR ("Double Reasoned Maturing"): innovative technique of agronomic ecologic control of grey mould on grapevine. Trials and various considerations. Commun. Agric. Appl. Biol. Sci. 71, 1055–1061.

  • Castillo-Muńoz, N., Gómez-Alonso, S., García-Romero, E., and Hermosín-Gutierréz, I. (2007). Flavonol profiles of Vitis vinifera red grapes and their single-cultivar wines. J. Agric. Food Chem. 55, 992–1002.

  • Conde, C., Silva, P., Fontes, N., Dias, A.C.P., Tavares, R.M., Sousa, M.J., Agasse, A., Delrot, S., and Gerós, H. (2007). Biochemical changes throughout grape berry development and fruit and wine quality. Food 1, 1–22.

  • Corso, M., Ziliotto, F., Rizzini, F.M., Teo, G., Cargnello, G., and Bonghi, C. (2013). Sensorial, biochemical and molecular changes in 'Raboso Piave' grape berries applying "Double Maturation Raisonnée" and late harvest techniques. Plant Sci. 208, 50–57.

  • De Lorenzis, G., Imazio, S., Rusjan, D., Vouillamoz, J.F., Nikolaou, N., Failla, O., and Scienza, A. (2013). Genetic investigation of grapevine varieties ‘Ribolla Gialla’ (Italy), ‘Rebula’ (Slovenia) and ‘Robola’ (Ionian Islands). Sci. Hortic. 150, 425–431.

  • del Llaudy, M.C., Canals, R., Canals, J.M., and Zamora, F. (2008). Influence of ripening stage and maceration length on the contribution of grape skins, seeds and stems to phenolic composition and astringency in wine-simulated macerations. Eur. Food Res. Technol. 226, 337–344.

  • Deluc, L.G., Quilici, D.R., Decendit, A., Grimplet, J., Wheatley, M.D., Schlauch, K.A., Mérillon, J.M., Cushman, J.C., and Cramer, G.R. (2009). Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of 'Cabernet Sauvignon' and 'Chardonnay'. BMC Genomics 10, 212.

  • Fanzone, M., Zamora, F., Jofré, V., Assof, M., and Pe-a-Neira, Á. (2011). Phenolic composition of 'Malbec' grape skins and seeds from Valle de Uco (Mendoza, Argentina) during ripening. Effect of cluster thinning. J. Agric. Food Chem. 59, 6120–6136.

  • Garrido, J., and Borges, F. (2013). Wine and grape polyphenols – A chemical perspective. Food Res. Int. 54, 1844–1858.

  • Gil, M., Esteruelas, M., González, E., Kontoudakis, N., Jiménez, J., Fort, F., Canals, J.M., Hermosín-Gutiérrez, I., and Zamora, F. (2013). Effect of two different treatments for reducing grape yield in Vitis vinifera cv. ‘Syrah’ on wine composition and quality: Berry thinning versus cluster thinning. J. Agric. Food Chem. 61, 4968–4978.

  • Hernandéz-Hierro, J.M., Quijada-Morín, N., Martínez-Lapuente, L., Guadalupe, Z., Ayestarán, B., Rivas-Gonzalo, J.C., and Escribano-Bailón, M.T. (2014). Relationship between skin cell wall composition and anthocyanin extractability of Vitis vinifera L. cv. ‘Tempranillo’ at different grape ripeness degree. Food Chem. 146, 41–47.

  • Jackson, D.I., and Lombard, P.B. (1993). Environmental and management practices affecting grape composition and wine quality – A review. Am. J. Enol. Vitic. 44, 409–430.

  • János, M., Gizella, J., Csaba, N., Gyӧnghi, K.S., and Péter, V. (2007). Application of a special grape growing method the DMR for the production of traditional wine specialities in Badacsony. Proceedings of the XXXth OIV World Congress (Budapest, Hungary), p.1–6.

  • Jeffrey, D., Parker, M., and Smith, P. (2008). Flavonol composition of Australian red and white wines determined by high‐performance liquid chromatography. Aust. J. Grape Wine Res. 14, 153–161.

  • Jones, G.V., and Davis, R.E. (2000). Climate influences on grapevine phenology, grape composition, and wine production and quality for Bordeaux, France. Am. J. Enol. Vitic. 51, 249–261.

  • Koruza, B., Vaupotič, T., Škvarč, A., Korošec-Koruza, Z., and Rusjan, D. (2012). Catalogue of Slovenian grapevine clones (Nova Gorica, Slovenia: Chamber of Agriculture and Forestry).

  • Koundouras, S., Marinos, V., Gkoulioti, A., Kotseridis, Y., and Van Leeuwen, C. (2006). Influence of vineyard location and vine water status on fruit maturation of nonirrigated cv. 'Agiorgitiko' (Vitis vinifera L.). Effects on wine phenolic and aroma components. J. Agric. Food Chem. 54, 5077–5086.

  • Landrault, N., Poucheret, P., Ravel, P., Gasc, F., Cros, G., and Teissedre, P.L. (2001). Antioxidant capacities and phenolics levels of French wines from different varieties and vintages. J. Agric. Food Chem. 49, 3341–3348.

  • Makris, D.P., Kallithraka, S., and Kefalas, P. (2006). Flavonols in grapes, grape products and wines: Burden, profile and influential parameters. J. Food Compos. Anal. 19, 396–404.

  • Mazza, G., Fukumoto, L., Delaquis, P., Girard, B., and Ewert, B. (1999). Anthocyanins, phenolics, and color of 'Cabernet Franc', 'Merlot', and 'Pinot Noir' wines from British Columbia. J. Agric. Food Chem. 47, 4009–4017.

  • Mencarelli, F., Bellincontro, A., Nicolleti, I., Cirilli, M., Muleo, R., and Corradini, D. (2010). Chemical and biochemical change of healthy phenolic fractions in winegrape by means of postharvest dehydration. J. Agric. Food Chem. 58, 7557–7564.

  • Mikulič-Petkovšek, M.M., Štampar, F., and Veberič, R. (2007). Parameters of inner quality of the apple scab resistant and susceptible apple cultivars (Malus domestica Borkh.). Sci. Hortic. 114, 37–44.

  • Mikulič-Petkovšek, M., Slatnar, A., Štampar, F., and Veberič, R. (2012). HPLC–MSn identification and quantification of flavonol glycosides in 28 wild and cultivated berry species. Food Chem. 135, 2138–2146.

  • Ministry of Agriculture, Forestry and Nutrition (2004). Rules on the requirements for grapes to be used for processing into wine, authorized oenological practices and processes used in the production of wine, and quality conditions for must and wine and other products in circulation.

  • Monagas, M., Bartolomé, B., and Gómez-Cordovés, C. (2005). Updated knowledge about the presence of phenolic compounds in wine. Crit. Rev. Food Sci. Nutr. 45, 85–118.

  • Ough, C., and Amerine, M. (1988). Acidity and Individual Acids. Methods for Analysis of Musts and Wine, 2nd ed (New York, NY, USA: Wiley), p.50–70.

  • Panceri, C.P., Gomes, T.M., De Gois, J.S., Borges, D.L.G., and Bordignon-Luiz, M.T. (2013). Effect of dehydration process on mineral content, phenolic compounds and antioxidant activity of 'Cabernet Sauvignon' and 'Merlot' grapes. Food Res. Int. 54, 1343–1350.

  • Peršurić, Đ., Cargnello, G., and Bratović, I. (2000). Impact of partly defoliation on must and wine quality of 'Chardonnay' variety on the short cut cordon. Prospects for Viticulture and Enology (Zagreb, Croatia), p.22–24.

  • Prajitna, A., Dami, I.E., Steiner, T.E., Ferree, D.C., Scheerens, J.C., and Schwartz, S.J. (2007). Influence of cluster thinning on phenolic composition, resveratrol, and antioxidant capacity in 'Chambourcin' wine. Am. J. Enol. Vitic. 58, 346–350.

  • Río Segade, S., Soto Vázquez, E., and Díaz Losada, E. (2008). Influence of ripeness grade on accumulation and extractability of grape skin anthocyanins in different cultivars. J. Food Compos. Anal. 21, 599–607.

  • Rolle, L., Caudana, A., Giacosa, S., Gerbi, V., and Río Segade, S. (2011). Influence of skin hardness on dehydration kinetics of wine grapes. J. Sci. Food Agric. 91, 505–511.

  • Rolle, L., Torchio, F., Giacosa, S., and Gerbi, V. (2009). Modifications of mechanical characteristics and phenolic composition in berry skins and seeds of 'Mondeuse' winegrapes throughout the on-vine drying process. J. Sci. Food Agric. 89, 1973–1980.

  • Romero-Pérez, A.I., Ibern-Gómez, M., Lamuela-Raventós, R.M., and De la Torre-Boronat, M.C. (1999). Piceid, the major resveratrol derivative in grape juices. J. Agric. Food Chem. 47, 1533–1536.

  • Romero-Pérez, A.I., Lamuela-Raventós, R.M., Andrés-Lacueva, C., and De la Torre-Boronat, M.C. (2001). Method for the quantitative extraction of resveratrol and piceid isomers in grape berry skins. Effect of powdery mildew on the stilbene content. J. Agric. Food Chem. 49, 210–215.

  • Rusjan, D., Korošec-Koruza, Z., and Veberič, R. (2008). Primary and secondary metabolites related to the quality potential of table grape varieties (Vitis vinifera L.). Eur. J. Hortic. Sci. 73, 124–130.

  • Rusjan, D., Veberič, R., and Mikulič-Petkovšek, M. (2012). The response of phenolic compounds in grapes of the variety 'Chardonnay' (Vitis vinifera L.) to the infection by phytoplasma Bois noir. Eur. J. Plant Pathol. 133, 965–974.

  • Ružić, I., Škerget, M., Knez, Ž., and Runje, M. (2011). Phenolic content and antioxidant potential of macerated white wines. Eur. Food Res. Technol. 233, 465–472.

  • Singleton, V.L., Orthofer, R., and Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299, 152–178.

  • Slovenian Environment Agency (2016). State Meteorological Service, Ministry of Environment and Spatial Planning of Slovenia.

  • Šuklje, K., Baša Česnik, H., Janeš, L., Kmecl, V., Vanzo, A., Deloire, A., Sivilotti, P., and Lisjak, K. (2013). The effect of leaf area to yield ratio on secondary metabolites in grapes and wines of Vitis vinifera L. cv. 'Sauvignon Blanc'. J. Int. Sci. Vigne Vin 47, 83–97.

  • Tourtoglou, C., Nenadis, N., and Paraskevopoulou, A. (2014). Phenolic composition and radical scavenging activity of commercial Greek white wines from Vitis vinifera L. cv. 'Malagousia'. J. Food Compos. Anal. 33, 166–174.

  • Wang, S.Y., Zheng, W., and Galletta, G.J. (2002). Cultural system affects fruit quality and antioxidant capacity in strawberries. J. Agric. Food Chem. 50, 6534–6542.

Received: 13 September 2016 | Accepted: 22 November 2016 | Published: 23 December 2016 | Available online: 23 December 2016

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