Eur.J.Hortic.Sci. 81 (6) 297-302 | DOI: 10.17660/eJHS.2016/81.6.2|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article
Accumulation of anthocyanin in apples in response to blue light at 450 nm: recommendations for producing quality fruit color under global warming
O. Arakawa, S. Kikuya, P. Pungpomin, S. Zhang and N. Tanaka
Faculty of Agriculture and Life Science, Hirosaki University, Japan
New apple (Malus domestica Borkh.) cultivars that can accumulate anthocyanin under nondirective visible light (without UV) and high temperatures will produce apples of good color under global warming conditions. However, how cultivars respond to visible light in terms of anthocyanin production and the role visible light plays in red color development have yet to be thoroughly studied. Accordingly, this study determined the effect of blue light on anthocyanin accumulation in two apple cultivars, 'Jonathan' and 'Fuji', under different temperatures. The results show that blue light of around 450 nm was most effective on anthocyanin accumulation in ‘Jonathan’ apples. This cultivar produced high amounts of anthocyanin and developed a dark red color under light-emitting diode (LED) blue light at 450 nm at temperatures between 15°-25°C. Even under temperatures as high as 30°C, blue light stimulated anthocyanin production, while its amount was low. These results suggest that this cultivar’s trait of responding to blue light at 450 nm could play a regulatory role in developing red color under nondirective visible light conditions, leading to the hypothesis that it may be possible to select new cultivars that easily develop red color under conditions of global warming.
anthocyanin, blue light, LED, red color
Significance of this study
What is already known on this subject?
What are the new findings?
The effective visible light region on color development in apple fruit had been studied, suggesting that blue light in the region of 450-480 nm and red light at the wavelength of 650 nm produced red color in apples. However, the most effective wavelength in the visible light region had not been shown, and the role of visible region on red color development remains unclear.
What is the expected impact on horticulture?
The action spectrum showed that the region around 450 nm was most effective for anthocyanin production in fruit of cv. 'Jonathan'. Under 450 nm this cultivar produced high amounts of anthocyanin and developed a dark red color at temperatures between 15°-25°C. In 'Fuji' fruit, the effect of blue light on anthocyanin accumulation and red color development was very less promotive. Results suggest that blue light (450 nm) induces to good apple coloration.
From the present results we propose that response to blue light at 450 nm contributes to good coloration under conditions of global warming. Our results can help breeders and growers select cultivars that easily develop a red color under such conditions. The blue light identified in this study may be useful for testing cultivar traits for fruit color development.
Arakawa, O., Hori, Y., and Ogata, R. (1985). Relative effectiveness and interaction of ultraviolet-B, red and blue light in anthocyanin synthesis of apple fruit. Physiol. Plant. 64, 323–327. https://doi.org/10.1111/j.1399-3054.1985.tb03347.x.
Arakawa, O. (1988). Characteristics of color development in some apple cultivars: Changes in anthocyanin synthesis during maturation as affected by bagging and light quality. J. Jpn. Soc. Hortic. Sci. 57, 373–380. https://doi.org/10.2503/jjshs.57.373.
Arakawa, O. (1991). Effect of temperature on anthocyanin accumulation in apple fruits as affected by cultivar, stage of fruit ripening, and bagging. J. Hortic. Sci. 66, 763–768. https://doi.org/10.1080/00221589.1991.11516209.
Bishop, R.C., and Klein, R.M. (1975). Photo-promotion of anthocyanin synthesis in harvested apples. Hortic. Sci. 10, 126–127.
Faragher, J.D. (1983). Temperature regulation of anthocyanin accumulation in apple skin. J. Exp. Botany 34, 1921–1928. https://doi.org/10.1093/jxb/34.10.1291.
Felicetti, D.A., and Schrader, L.E. (2008). Changes in pigment concentrations associated with the degree of sunburn browning of 'Fuji' apple. J. Amer. Soc. Hortic. Sci. 133, 27–34.
Kadomura-Ishikawa, Y., Miyawaki, K., Noji, S., and Takahashi, A. (2013). Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria × ananassa fruits. J. Plant. Res. 126, 847–857. https://doi.org/10.1007/s10265-013-0582-2.
Lancaster, J.E. (1992). Regulation of skin color in apples. Crit. Rev. Plant Sci. 10, 487–502. https://doi.org/10.1080/07352689209382324.
Li, Y., Mao, K., Zhao, C., Zhao, X., Zhang, R., Zhang, H., Shu, H., and Hao, Y. (2013). Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica). Plant Cell Rep. 32, 555–566. https://doi.org/10.1007/s00299-013-1387-4.
Pearce, G.W., and Streeter, R. (1931). A report on the effect of light on pigment formation in apples. J. Biol. Chem. 92, 743–749.
Richard, H.G. (1997). Partitioning of biologically active radiation in plant canopies. Int. J. Biometeorol. 40, 26–40. https://doi.org/10.1007/BF02439408.
Schultz, H.R. (2000). Climate change and viticulture: A European perspective on climatology, carbon dioxide and UV-B effects. Aust. J. of Grape and Wine Res. 6, 2–12. https://doi.org/10.1111/j.1755-0238.2000.tb00156.x.
Siegelman, H.W., and Hendricks, S.B. (1959). Photocontrol of anthocyanin synthesis in apple skin. Plant Physiol. 33, 185–190. https://doi.org/10.1104/pp.33.3.185.
Sugiura, T., and Yokozawa, M. (2004). Impact of global warming on environments for apple and satsuma mandarin production estimated from changes of the annual mean temperature. J. Jpn. Soc. Hortic. Sci. 73, 72–78. https://doi.org/10.2503/jjshs.73.72.
Ubi, B., Honda, C., Bessho, H., Kondo, S., Wada, M., Kobayashi, S., and Moriguchi, T. (2006). Expression analysis of anthocyanin biosynthetic genes in apple skin: effect of UV-B and temperature. Plant Sci. 170, 571–578. https://doi.org/10.1016/j.plantsci.2005.10.009.
Received: 6 September 2016 | Accepted: 22 November 2016 | Published: 23 December 2016 | Available online: 23 December 2016