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  Eur.J.Hortic.Sci. 81 (5) 273-280 | DOI: 10.17660/eJHS.2016/81.5.6
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article

Mushroom: molecular characterization of indigenous species in the Niger Delta Region of Nigeria

O.M. Adedokun1, M. Kyalo2, B. Gnonlonfin2,3,4,5, J. Wainaina2,6, D. Githae2, R. Skilton2,7 and J. Harvey2,8
1University of Port-Harcourt, Department of Crop and Soil Science, Faculty of Agriculture, Rivers State, Nigeria
2Biosciences Eastern and Central Africa – International Livestock Research Institute Hub, Nairobi, Kenya
3Catholic University of Eastern Africa, Nairobi, Kenya
4Commonwealth Scientific and Industrial Research Organisation, Australia
5National Institute of Agricultural Research of Benin, Cotonou, Benin
6The University of Western Australia, School of Chemistry and Biochemistry, Australia
7International Centre of Insect Physiology and Ecology (ICIPE), Nairobi, Kenya
8Kansas State University, Manhattan, Kansas 66506, USA

Background of the study: Food and nutritional security remain a great challenge especially in developing countries. To address this challenge the exploration of natural resources such as mushrooms should be considered. Mushrooms species can be morphologically similar but biochemically different, hence the need for accurate identification. Objective: The objective of this study was to identify indigenous mushrooms in Niger Delta region of Nigeria using molecular tools (ITS barcoding). Methods: Mushroom samples (N=57) were collected across six states of the Niger Delta Region: Abia, Akwa Ibom, Bayelsa, Delta, Imo and Rivers. ITS DNA barcoding was used for species identification. The ITS1, 5.8S rDNA and ITS2 locus was amplified. Results: Nucleotide sequences analysis revealed genetic diversity among samples with 57 isolates belonging to 27 species of 14 different families identified. The family Polyporaceae was the most abundant comprising five species, 23 isolates with 40.4%, followed by Pleurotaceae comprising five species, ten isolates with 17.5 %. Conclusion: Indigenous mushrooms in The Niger Delta Region of Nigeria are diverse. ITS barcoding was effective in identification of samples. This study will promote knowledge of the diverse species. As a result, it will enhance cultivation and consumption of indigenous wild mushrooms and also will improve livelihoods for small holder farmers.

Keywords barcoding, biodiversity, indigenous wild mushroom, ITS, Niger Delta Region, species identification

Significance of this study

What is already known on this subject?

  • The Niger Delta Region of Nigeria harbors diverse mushroom species in the wild. Usage of these mushrooms is limited because of inadequate identification research and documentation, thus the public is not well informed as to benefits derivable from mushrooms.
What are the new findings?
  • ITS barcoding was effective to identify most species of the mushrooms collected across the Niger Delta Region. This study is unique in that it describes molecular characterization of mushrooms in Nigeria on which there is limited available literature.
What is the expected impact on horticulture?
  • Molecular identification of these mushrooms gives insight to their usage. Detailed research on domestication and consumption is expected to be higher, thus contributing to food security and bridging the gap of malnutrition, hidden hunger and mushroom phobia.

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Received: 10 June 2016 | Accepted: 16 September 2016 | Published: 30 October 2016 | Available online: 26 October 2016

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