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  Eur.J.Hortic.Sci. 81 (4) 212-218 | DOI: 10.17660/eJHS.2016/81.4.4
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article

Effects of gamma ray dose rate and sucrose treatment on mutation induction in chrysanthemum

Ye-Sol Kim1, Sang Yeop Sung1, Yeong Deuk Jo1, Hyo-Jeong Lee2 and Sang Hoon Kim1
1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea
2Crop Breeding Division, National Institute of Crop Science, RDA, Wanju 55365, Korea

Gamma rays are useful to develop new cultivars for mutation breeding, in which the total dose and dose rate are important. On two chrysanthemum cultivars, 'Noble Wine' and 'Pinky', were tested the effect of the dose rate. Four weeks after irradiation, their survival rate and plant height were significantly decreased at the dose rate 30 Gy/1 h. The dose rate 30 Gy/4 h produced the highest mutation frequency in both cultivars, with a wide mutation spectrum. Moreover, sucrose is known as stimulus of anthocyanin biosynthesis. For the induction of anthocyanin related flower-color mutants, we pre-treated 4-week old plants with 100, 200 and 300 mM sucrose before gamma irradiation. The cultivar 'Pinky' showed a high survival rate in all treatments; while almost all 'Noble Wine' plants treated with 200 and 300 mM sucrose died. A number of mutated branches were identified at 100 mM sucrose + 30 Gy/24 h of irradiation in the cultivar 'Noble Wine'. Thus, a proper dose rate of gamma rays and sucrose pre-treatment could induce flower color mutation in chrysanthemum.

Keywords anthocyanin biosynthesis, mutation breeding, mutation frequency

Significance of this study

What is already known on this subject?

  • Mutation breeding is widely used to develop new cultivars including flower color mutants. Especially gamma rays are one of the important mutagens. To determine proper dose and dose rate is important to induce mutation using gamma rays. Furthermore, sucrose is a stimulator of anthocyanin biosynthesis. When target genes are highly expressed, irradiation is more effective.
What are the new findings?
  • Survival rate and plant height were decreased with increasing dose rate, particularly in the cultivar 'Pinky'. Plants irradiated with 30 Gy for 4 h showed a high mutation frequency with a wide color spectrum in both cultivars. In addition, irradiated plants with 100 mM sucrose pre-treatment induced many flower color mutants however, most all plant wither on 200 mM and 300 mM of sucrose treatment in the cultivar 'Noble Wine'.
What is the expected impact on horticulture?
  • Based on these results, it will help research suitable dose rate in gamma ray mutagenesis with various mutation spectrum in chrysanthemum. A combination of sucrose pre-treatment and gamma irradiation is effective to induce mutants. It would encourage to develop new cultivars using gamma rays.

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Received: 26 April 2016 | Accepted: 1 July 2016 | Published: 29 August 2016 | Available online: 29 August 2016

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