Eur.J.Hortic.Sci. 81 (3) 137-147 | DOI: 10.17660/eJHS.2016/81.3.1|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article
Phenotypic and molecular effects of chronic gamma irradiation on Curcuma alismatifolia
S. Taheri1, T.L. Abdullah1, Z. Ahmad2, M. Sahebi3,4 and P. Azizi5
1Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia
2Agrotechnology and Biosciences Division, Malaysian Nuclear Agency, Bangi, Selangor, Malaysia
3Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
4Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
5Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
Mutation breeding is one of the methods for generating genetic variation and obtaining new cultivars of ornamental plants during the past decades. In present study, the effects of four doses (0, 14.6, 33, and 87.4 Gy) of chronic gamma irradiation on three cultivars of Curcuma alismatifolia and one Curcuma hybrid were investigated. Morphological aberrations from non-treated plants were observed by exposing growing plants. Higher doses induced phenotypical variations and significantly affected the plant growth parameters and flowering capacity. In terms of genetic variation, among the irradiated cultivars, the number of presumed alleles revealed by SSR analysis ranged from two to five with a mean value of 3.1 to 3.7 alleles per locus for radiation doses. The average value of the effective number of alleles, Neis gene diversity, and Shannons information index were 2.422.66, 0.500.56, and 0.901.03, respectively. Heat map hierarchical clustering divided 52 studied individuals into four major clusters. Results of this study showed that chronic gamma irradiation efficiently can enhance the phenotypical and genetic variations in C. alismatifolia cultivars at doses of 33 Gy and 84.6 Gy. In addition, SSR markers will likely accelerate the progress of selection of desired mutants during mutation breeding programs.
genetic variation, mutation breeding, microsatellite markers, morphological variation, Zingiberaceae
Significance of this study
What is already known on this subject?
What are the new findings?
Mutation breeding is one of the most effective ways for development of novel ornamental plants and crop varieties and increasing genetic and phenotypic variation.
What is the expected impact on horticulture?
The results obtained from this study include finding of optimum doses of chronic gamma irradiation, production of desired bract and leaf color and shape of Curcuma alismatifolia. In addition, SSR markers were used for induced genetic variation detection.
The topic could be of great interest for horticulturists dealing with mutation breeding and development of new cultivars of ornamental plants, since chronic gamma irradiation has the potential for development of new variants of Curcuma alismatifolia as pot plant or cut flower to promote the flower industry.
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Ahloowalia, B.S., and Maluszynski, M. (2001). Induced mutations A new paradigm in plant breeding. Euphytica 118, 167173. https://doi.org/10.1023/A:1004162323428.
Acquaah, G. (2007). Principles of plant genetics and breeding (Oxford: Blackwell), pp. 385.
Albashir, M. (2004). Effect of gamma irradiation on fungal load, chemical and sensory characteristics of walnut. Stored Products Res. 40, 355362. https://doi.org/10.1016/S0022-474X(03)00030-4.
Azhar, M., and Ahsanulkhaliqin, A.W. (2014). Gamma greenhouse: A chronic facility for crops improvement and agrobiotechnology. Paper presented at AIP Conference, Kuala Lumpur, Malaysia, 30 September2 October 2013, pp. 3237. https://doi.org/10.1063/1.4866100.
Banerji, B.K., and Datta, S.K. (1992). Gamma ray induced flower shape mutation in Chrysanthemum cv. Jaya. J. Nucl. Agric. Biol. 21, 7379.
Das, A., Kesari, V., Satyanarayana, V.M., Parida, A., and Rangan, L. (2011). Genetic relationship of Curcuma species from northeast India using PCR-Based markers. Mol. Biotechnol. 49, 6576. https://doi.org/10.1007/s12033-011-9379-5.
Dogbevi, M.K., Vachon, C., and Lacroix, M. (2000). Effect of gamma irradiation on the microbiological quality and on the functional properties of proteins in dry red kidney beans (Phaseolus vulgaris). Radiat. Phys. Chem. 57, 265268. https://doi.org/10.1016/S0969-806X(99)00442-9.
Fu, H.W., Li, Y.F., and Shu, Q.Y. (2008). A revisit of mutation induction by gamma rays in rice (Oryza sativa L.): implications of microsatellite markers for quality control. Mol. Breed. 22, 281288. https://doi.org/10.1007/s11032-008-9173-7.
Gastel, A.J.G., and de Nettancourt, D.D. (1974). The effects of different mutagens on self-incompatibility in Nicotiana alata Link and Otto: I. Chronic gamma irradiation. Radiat. Bot. 14, 4350. https://doi.org/10.1016/S0033-7560(74)90096-9.
Jiang, G.L. (2013). Molecular markers and marker-assisted breeding in plants. Plant breeding from laboratories to fields. InTech. https://doi.org/10.5772/52583.
Kadkhodaei, S., Shahnazari, M., Khayyam, Nekouei, M., Ghasemi, M., Etminani, H., and Imani, A. (2011). A comparative study of morphological and molecular diversity analysis among cultivated almonds (Prunus dulcis). Aust. J. Crop Sci. 5, 8291.
Kang, S.Y., Kim, J.B., and Lee, G.J. (2010). Construction of phytotron: New chronic irradiation facility using 60C gamma-ray. Plant Mutation Reports 2, 5254.
Kemp, S.J. (2002). PIC calculator. http://www.liv.ac.uk/~kempsj/pic.html.
Klein, R.M., and Klein, D.T. (1971). Post-irradiation modulation of ionizing radiation damage to plants. Botanic. Rev. 37(4), 397436. https://doi.org/10.1007/BF02868684.
Kovacs, E., and Keresztes, A. (2002). Effect of gamma and UV-B/C radiation on plant cells. Micron 33, 199210. https://doi.org/10.1016/S0968-4328(01)00012-9.
Kovalchuk, O., Arkhipov, A., Barylyak, I., Karachov, I., Titov, V., Hohn, B., and Kovalchuk, I. (2000). Plants experiencing chronic internal exposure to ionizing radiation exhibit higher frequency of homologous recombination than acutely irradiated plants. Mutat. Res. 449, 4756. https://doi.org/10.1016/S0027-5107(00)00029-4.
Lamseejan, S., Jompuk, P., Wongpiyasatid, A., Deeseepan, S., and Kwanthammachart, P. (2000). Gamma-rays induced morphological changes in Chrysanthemum (Chrysanthemum morifolium). Kasetsart J. (Nat. Sci.) 34, 417422.
Liao, M., Wang, Y., Rong, X., Zhang, Z., Li, B., Wang, L., and Chen, G. (2011) Development of new microsatellite DNA markers fromApostichopus japonicus and their cross-species application in Parastichopus parvimensis and Pathallus mollis. Int. J. Mol. Sci. 12, 58625870. https://doi.org/10.3390/ijms12095862.
Mac, C., Teoh, S.B., and Ratnam, A. (1986). The influence of gamma-rays on the injury and chromosomal aberrations of long bean (Vigna sesquipedalis Fruw.). Pertanika 9, 109117.
Mahadtanapuk, S. (2012). Quality improvement of Curcuma flowers by molecular biology technology. Naresuan Phayao J. 5(1), 111.
Meiselman, N., Gunckel, J.E., and Sparrow, A.H. (1961). The general morphology and growth responses of two species of Nicotina and their interspecific hybrid after chronic gamma irradiation. Radiat. Bot. 1, 6979. https://doi.org/10.1016/S0033-7560(61)80008-2.
Nei, M. (1973). Analysis of gene diversity in subdivided populations. Paper presented at National Academy Sciences of the United States of America, 1st December, 1973, pp. 33213323. https://doi.org/10.1073/pnas.70.12.3321.
Paisooksantivatana, Y., and Thepsen, O. (2001). Phenetic relationship of some Thai Curcuma species (Zingiberaceae) based on morphological, palynological and cytological evidence. Thai J. Agric. Sci. 34, 4757.
Peakall, R., and Smouse, P.E. (2012). GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research an update. Bioinformatics 28, 25372539. https://doi.org/10.1093/bioinformatics/bts460.
Quastler, H., Schertiger, A.M., and Stewart, W.N. (1952). Inhibition of growth by irradiation. IV. Growth arrest vs. effects on mitotic activity. J. Cell Comp. Physiol. 39, 357369. https://doi.org/10.1002/jcp.1030390303.
R Core Team. (2014). R: A language and environment for statistical computing (Vienna, Austria: R Foundation for Statistical Computing). URL http://www.R-project.org/.
Ramachandran, M., and Goud, J.V. (1983). Mutagenesis in safflower (Carthamus tinctorius). Differential radiosensitivity. Genet. Agraria 37, 309318.
Rohlf, F.J. (2002). NTSYS-pc: Numerical taxonomy system, version 2.1. (NY, USA: Exeter Publishing, Ltd.).
Ruamrungsri, S., Ohtake, N., Sueyoshi, K., and Ohyama, T. (2006). Determination of the uptake and utilization of nitrogen in Curcuma alismatifolia Gagnep. using 15N isotope. Soil Sci. Plant Nutr. 52(2), 221225. https://doi.org/10.1111/j.1747-0765.2006.00027.x.
Rusli, I. (2010). Malaysian Nuclear Agency Gamma Greenhouse. In Plant Mutation Report, Q. Shu, ed. (Vienna: IAEA), pp. 5254
Shikazono, N., Yokota, Y., Kitamura, S., Suzuki, C., Watanabe, H., Tano, S., and Tanaka, A. (2003). Mutation rate and novel tt mutants of Arabidopsis thaliana induced by carbon ions. Genetics 163, 14491455.
Shiran, B., Amirbakhtiar, N., Kiani, S., Mohammadi, S.H., Sayed-Tabatabaei, B.E., and Moradi, H. (2007). Molecular characterization and genetic relationship among almond cultivars assessed by RAPD and SSR markers. Scientia Hortic. 111, 280292. https://doi.org/10.1016/j.scienta.2006.10.024.
Shukla, R., and Datta, S.K. (1993). Mutation studies on early and late varieties of garden Chrysanthemum. J. Nucl. Agric. Biol. 22, 138142.
Sigrist, M.S., Pinheiro, J.B., Azevedo-Filho, J.A., Colombo, C.A., Sandhu, S., Souza, A.P., and Zucchi, M.I. (2010). Development and characterization of microsatellite markers for turmeric (Curcuma longa L.). Plant Breed. 129, 570573.
Sigrist, M.S., Pinheiro, J.B., Filho, J.A., and Zucchi, M.I. (2011). Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identified by microsatellite markers. Genet. Mol. Res. 10, 419428. https://doi.org/10.4238/vol10-1gmr1047.
Siju, S., Dhanya, K., Syamkumar, S., Sheeja, T.E., Sasikumar, B., Bhat, A.I., and Parthasarathy, V.A. (2010). Development, characterization, and utilization of genomic microsatellite markers in turmeric (Curcuma longa L.). Biochem. Sys. Ecol. 38, 641646. https://doi.org/10.1016/j.bse.2010.08.006.
Singh, S., Panda, M.K., and Nayak, S. (2012). Evaluation of genetic diversity in turmeric (Curcuma longa L.) using RAPD and ISSR markers. Ind. Crops Prod. 37, 284291. https://doi.org/10.1016/j.indcrop.2011.12.022.
Skornickova, J. (2006). Curcuma-stunning beauty, hidden treasure. Gardenwise 27, 44.
Sparrow, A.H. (1951). Radiation sensitivity of cells during mitotic and meiotic cycles with emphasis on possible cytochemical changes. Annals N. Y. Acad. Sci. 51, 15081540. https://doi.org/10.1111/j.1749-6632.1951.tb30076.x.
Sparrow, A.H., and Singleton, W.R. (1953). The use of radio-cobalt as a source of gamma rays and some effects of chronic irradiation on growing plants. Amer. Nat. 117, 2948. https://doi.org/10.1086/281753.
Sung, W.C. (2005). Effect of gamma irradiation on rice and its food products. Radiat. Phys. Chem. 73, 224228. https://doi.org/10.1016/j.radphyschem.2004.08.008.
Taheri, S., Abdullah, T.L., Abdullah, N.A.P., and Ahmad, Z. (2013). Use of intersimple sequence repeat assay for detection of DNA Polymorphism induced by gamma rays in Curcuma alismatifolia. HortScience 48, 13461351.
Taheri, S., Abdullah, T.L., Ahmad, Z., and Abdullah, N.A.P. (2014). Effect of acute gamma irradiation on Curcuma alismatifolia varieties and detection of DNA polymorphism through SSR marker. BioMed. Res. Int. 2014, 118. https://doi.org/10.1155/2014/631813.
Taheri, S. (2014). Phenotypic and molecular variation among selected Curcuma alismatifolia Gagnep. mutants derived from acute and chronic gamma irradiation (Unpublished doctoral dissertation). Universiti Putra Malaysia.
Tangpong, P., Taychasinpitak, T., Jompuk, C., and Jompuk, P. (2009). Effects of acute and chronic gamma irradiations on In vitro culture of Anubias congensis N.E. Brown. Kasetsart J. (Nat. Sci.) 43, 449457.
Thohirah, L.A., Flora, C.L.S., and Kamalakshi, N. (2010). Breaking bud dormancy and different shade levels for production of pot and cut Curcuma alismatifolia. Am. J. Agric. Biol. Sci. 5, 385388. https://doi.org/10.3844/ajabssp.2010.385.388.
Voisine, R., Vezina, L.P., and Willemot, C. (1991). Induction of senescence-like deterioration of microsomal membranes from cauliflower by free radicals generated during gamma irradiation. Plant Physiol. 97, 545550. https://doi.org/10.1104/pp.97.2.545.
Wi, S.G., Chung, B.Y., Kim, J.H., Baek, M.H., Yang, D.H., Lee, J.W., and Kim, J.S. (2005). Ultrastructural changes of cell organelles in Arabidopsis stems after gamma irradiation. J. Plant Biol. 48, 195200. https://doi.org/10.1007/BF03030408.
Wi, S.G., Chung, B.Y., Kim, J.S., Kim, J.H., Baek, M.H., Lee, J.W., and Kim, Y.S. (2007). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron 38, 553564. https://doi.org/10.1016/j.micron.2006.11.002.
Wu, D., Shu, Q., Wang, Z., and Xia, Y. (2002). Effect of gamma irradiation on starch viscosity and physicochemical properties of different rice. Radiat. Phys. Chem. 65, 7986. https://doi.org/10.1016/S0969-806X(01)00676-4.
Yeh, F.C., Yang, R., Boyle, T.B.J., Ye, Z., and Mao, J.X. (2000). POPGENE, the user-friendly shareware for population genetic analysis (Edmonton, Alberta, Canada: Molecular Biology and Biotechnology Center, University of Alberta).
Zaka, R., Chenal, C., and Misset, M.T. (2004). Effects of low doses of short-term gamma irradiation on growth and development through two generations of Pisum sativum. Sci. Total Environ. 320, 121129. https://doi.org/10.1016/j.scitotenv.2003.08.010.
Received: 10 November 2015 | Revised: 29 December 2015 | Accepted: 26 January 2016 | Published: 20 June 2016 | Available online: 20 June 2016