Eur.J.Hortic.Sci. 81 (1) 44-48 | DOI: 10.17660/eJHS.2016/81.1.6|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article
Seed viability tests for Acer pictum and A. rubrum
Q.Y. Ma, L. Chen, J. Hou, H.L. Liu and S.X. Li
The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing, China
Seed viability is commonly used to rapidly evaluate the seed quality, especially for the seeds with deep dormancies. To establish the experimental protocol that could obtain reliable seed viability for Acer pictum and A. rubrum, different treatments were examined in excised embryo and tetrazolium (TZ) tests. In the excised embryo test, it was found that sterilization could get a significantly higher viability for both species. No significant difference was observed between seed viability and seed germination percentage when the excised embryos were sterilized following the protocol established in this study, indicating it was appropriate for evaluation of seed quality of these two maples. In the TZ test, we examined the effect of GA3 and stratification treatment on seed viability for these two species. For seeds of A. pictum, no significant difference was detected between the viabilities of seeds merely rehydrated with water and those rehydrated with GA3 and stratification. Both protocols yielded seed viabilities that deviated insignificantly from the seed germination percentage. Thus, additional treatments other than water immersion were unnecessary to measure the seeds viability of A. pictum. By contrast, significant difference was observed for seed viabilities of A. rubrum under the above different rehydration methods. Only when seeds were rehydrated with GA3 and stratification after water immersion yielded seed viability that was insignificantly different from seed germination percentage. Therefore, besides water immersion, additional treatments were compulsory for seed viability evaluation of A. rubrum. In this study, we also found that the radicle tip of a high percentage of A. rubrum embryos could only be partially stained in the TZ test, and this scenario was not related to the embryo colors of A. rubrum.
Acer pictum, Acer rubrum, excised embryo test, seed viability, tetrazolium test
Significance of this study
What is already known on this subject?
What are the new findings?
Seed viability is commonly used to evaluate the seed quality rapidly, especially for the seeds that have deep dormancy. Tetrazolium (TZ) and excised embryo tests are widely adopted methods to measure the viability. However, ISTA only recommended optimized protocols for limited plant species and part of maple plants were included in.
What is the expected impact on horticulture?
During the excised embryo tests sterilization could get a significantly higher viability for both species. We also found that the radicle tip of a high percentage of A. rubrum embryos could only be partially stained in the TZ test, and this phenomenon was consistent between the yellow and green embryos.
It was important to evaluate seed viability exactly. It was necessary to establish reliable protocol by experiments for measuring seed viability that was uncovered by ISTA Rules.
Bao, J.P., Zhang, S.L., Cheng, X., Cui, Y.B., and Liu, N. (2010). Study on determining vigor of Pryusocalleryana decaisne and Cydonia oblonga Mill. seeds rapidly. Seed 2, 119−122.
Beresniewicz, M.M., Taylor, A.G., Goffinet, M.C., and Koeller, W.D. (1995). Chemical nature of a semipermeable layer in seed coats of leek, onion (Liliaceae), tomato and pepper (Solanaceae). Seed Sci. Tech. 23, 135−145.
Drewitz, J.J., and Ditomaso, J.M. (2004). Seed biology of jubatagrass (Cortaderia jubata). Weed Sci. 52, 525530. https://doi.org/10.1614/WS-03-081R.
Flores, P.C., Poggi, D., Garcνa, S.M., and Gariglio, N.F. (2011). Topographic tetrazolium testing of Black Walnut (Juglans nigra L.). Seed Sci. Tech. 39, 230235. https://doi.org/10.15258/sst.2011.39.1.23.
Gosling, P.G. (2004). Viability testing. In Seed conservation: Turning science into practice, R.D. Smith, J.B. Dickie, S.H. Linington, H.W. Pritchard, and R.J. Probert, eds. (Kew, UK: The Royal Botanic Gardens), 24, 445481.
Guo, B., Jiang, W.B., and Weng, M.L. (2010). Tree species in Acer and their application in landscape architecture. Acta Agr. Jiangxi, 22, 4952.
Hou, J., Liu, H.L., Ma, Q.Y., Wei, W.T., Yin, T.M., and Li, S.X. (2014). Development of dormancy in seeds of Sapium sebiferum (L.) Roxb. during maturation. Prop. Ornam. Plants 14, 1319.
ISTA, International Seed Testing Association (2013). International Rules for Seed Testing (Zurich, Switzerland).
Johann, H. (1942). Origin of the suberized semipermeable membrane in the caryopsis of maize. J. Agr. Res. 64, 275281.
Kotowski, F. (1927). Semipermeability of seed coverings and stimulation of seeds. Plant Phys. 2, 177186. https://doi.org/10.1104/pp.2.2.177.
Li, S.X., Mao, Y., and Jiang, Y.Y. (2012a). Detection method of seed germination percentage of Fraxinus chinensis. J. Northeast For. Univ. 40, 14.
Li, S.X., Gu, H.B., Mao, Y., Yin, T.M., and Gao, H.D. (2012b). Effects of tallow tree seed coat on seed germination. Journal of Forestry Research 23, 229233. https://doi.org/10.1007/s11676-011-0217-1.
O'Brien, S.D., and Johnston, M.E. (2004). Seed viability and dormancy mechanisms of Leucopogon melaleucoides Cunn. ex DC. (Epacridaceae). Seed Sci. Tech. 32, 510. https://doi.org/10.15258/sst.2004.32.1.02.
Phartyal, S.S., Thapliyal, R.C., Nayal, J.S., and Joshi, G. (2003). Assessment of viability of Acer caesium and Ulmus wallichiana seeds through the tetrazolium test. Seed Sci. Tech. 31, 691700. https://doi.org/10.15258/sst.2003.31.3.18.
Thornton, M.L. (1968). Seed dormancy in watermelon Citrullus vulgaris Shrad. Proceedings of the Association of Official Seed Analysis 58, 8084.
Vankus, V. (1997). The tetrazolium estimated viability test for seeds of native plants. In National Proceedings, Forest and Conservation Nursery, T.D. Landis and J.R. Thompson, tech. coords. (U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station), pp. 5762.
Webb, D.P., and Dumbroff, E.B. (1969). Factors influencing the stratification process in seeds of Acer saccharum. Can. J. Bot. 47, 15551563. https://doi.org/10.1139/b69-222.
Wu, J., Hou, J., Ma, Q.Y., and Li, S.X. (2013). Seed dormancy mechanism of four Acer species and the methods for dormancy breaking. J. Southwest For. Univ. 33, 4851.
Zeng, L., Fan, G.S., and Duan, X.M. (2007). Comparative experiment on rapid test method on seed viability of three species of Proteaceae. J. West China For. Sci. 36, 119120.
Zeng, Y.J., and Wang, Y.R. (2009). Methods of topographical tetrazolium testing for seed viability of Nitraria tangutorum Bobr. and N. sibirica Pall. Seed Sci. Tech. 37, 691698. https://doi.org/10.15258/sst.2009.37.3.16.
Zhou, J., and Wang, Y.R. (2012). Research advances in semi-permeable layer of seed. Acta Botanica Boreali-Occidentalia Sinica 32, 19281934.
Received: 11 April 2015 | Revised: 21 July 2015 | Accepted: 15 September 2015 | Published: 22 February 2016 | Available online: 22 February 2016