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  Eur.J.Hortic.Sci. 81 (1) 37-43 | DOI: 10.17660/eJHS.2016/81.1.5
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article

Analysis of genetic diversity of the apricot germplasm in the southern region of the Tianshan Mountains in Xinjiang, China using the ISSR technique

J. Liu, K. Liao, M. Nasir, S. Zhao, Q. Sun and X. Peng
Research Center for Xinjiang Characteristic Fruit Tree, Xinjiang Agricultural University, Urumqi, Xinjiang, China

SUMMARY
The apricot originated from Xinjiang, China, and therefore, the apricot germplasm of Xinjiang is very abundant. The aim of this study was to explore the genetic diversity and the genetic relationship among different apricot populations thriving in the southern region of the Tianshan Mountains in Xinjiang, as well as to provide reference for the protection and utilization of resources and breeding. The method of inter-simple sequence repeats (ISSR) molecular marker technology was employed in the present study. The experimental materials included 141 samples from the apricot germplasm, which were derived from seven regions in the South of Tianshan Mountains. A total of 459 polymorphic bands were amplified using 22 ISSR primers. The percentage of polymorphic loci ranged from 40.65%–66.67%. Among the 12 populations analyzed, the percentage of polymorphic loci, the observed number of alleles, the effective number of alleles, Nei’s genetic diversity, and Shannon information index were highest in the Yingjisha population, whereas the lowest values were observed in the Aheqi and Toukexun population. Genetic variation in the apricot germplasm in the South of the Tianshan Mountains in Xinjiang was mainly due to intra-population variability (69.37%), and to a lesser extent, inter-population variability (30.63%). The Shanshan population and the Turpan population showed the closest genetic relationship and the lowest degree of genetic variation, whereas the Aheqi population and the Hetian population presented the most distant genetic relationship and the highest degree of genetic variation.

Keywords molecular marker, polymorphic, alleles, genetic relationship, variation, population

Significance of this study

What is already known on this subject?

  • From this subject we already know the genetic diversity and the genetic relationship among different apricot populations thriving in the southern region of the Tianshan Mountains in Xinjiang.
What are the new findings?
  • A total of 459 polymorphic bands were amplified using 22 ISSR primers. The percentage of polymorphic loci ranged from 40.65%–66.67%. Among the 12 populations analyzed, the percentage of polymorphic loci, the observed number of alleles, the effective number of alleles, Nei’s genetic diversity, and Shannon information index were highest in the Yingjisha population, whereas the lowest values were observed in the Aheqi and Toukexun population. Genetic variation in the apricot germplasm in the South of the Tianshan Mountains in Xinjiang was mainly due to intra-population variability (69.37%), and to a lesser extent, inter-population variability (30.63%). The Shanshan population and the Turpan population showed the closest genetic relationship and the lowest degree of genetic variation, whereas the Aheqi population and the Hetian population presented the most distant genetic relationship and the highest degree of genetic variation.
What is the expected impact on horticulture?
  • By the study, we expected to provide reference for the protection and utilization of resources and breeding.

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E-mail: liaokang01@163.com  

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Received: 13 September 2014 | Revised: 8 May 2015 | Accepted: 17 November 2015 | Published: 22 February 2016 | Available online: 22 February 2016

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