Eur.J.Hortic.Sci. 81 (1) 13-21 | DOI: 10.17660/eJHS.2016/81.1.2|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article
Early frost reactions of different populations of Quercus robur L. and Tilia cordata Mill. in Germany
M. Selig and H. Bohne
Institute for Horticultural Production Systems, Section Woody Plant and Propagation Physiology, Leibniz Universität Hannover, Hannover, Germany
Local adapted tree populations underlie the risk of losing their adaptive traits by hybridization with trees of non-local populations. To prevent this hybridization the German government amended the Federal Nature Conservation Act (BNatschG) in 2010 and six defined areas of origin were arranged (BMU, 2012) for the use of plants in free nature. The transfer of propagation material between them is forbidden from March 1st, 2020. However, the grade of local adaptation in populations of woody species in Germany is not clearly demonstrated. The aim of this study was to investigate differences in early frost reactions among German populations of Quercus robur and Tilia cordata by artificial freezing of shoots at -15°C and -25°C in November 2011. The process of bud set was rated prior and frost damage, sugar, starch and proline concentration were measured after the freezing experiments. To estimate long term survival whole plants were frozen simultaneously and then cultivated to investigate their regeneration. We did not find differences in bud set and early frost hardiness of shoots between German populations of the target species. Differences in these traits only occurred for the included Hungarian population of Quercus robur with lower grade of bud set and higher frost damage while having the highest proline concentration. Physiological differences occurred among German populations of Tilia cordata with higher soluble sugar concentration of the most southern population (47.51°N). However, these physiological differences did not improve frost hardiness indicating phenology as the main factor for early frost hardiness in the current experiment. The ecological differences at the sites of origin of the used populations do not seem to be big enough to generate different early frost reactions in our experiment, counteracting ecological factors affecting the same biological parameter substitute each other or high phenotypic plasticity covers local adaptation.
adaptation, bud set, carbohydrates, index of injury, proline, provenances
Significance of this study
What is already known on this subject?
What are the new findings?
The adaptation of woody plant populations to local ecological conditions can lead to different abiotic stress reactions, e.g., early frost. These adaptive traits can be lost by hybridization with non-local populations. However, the magnitude of local adaptation in German populations of Quercus robur and Tilia cordata is not fully clear. Nevertheless from March 1st, 2020 the Federal Nature Conservation Act forbids the planting of populations outside their newly arranged defined areas of origin in free nature.
What is the expected impact on horticulture?
After transfer to the experimental site in Hannover and growth for seven months, we did not find differences in bud set between German populations of the target species. Also after artificial freezing at -15°C and -25°C in November 2011 no interpopulation differences in frost damage occurred. Differences in sugar, starch and proline concentration were marginal.
Based on the results for possible risks due to early frost, the partitioning of six defined areas of origin for the use of woody plants in free nature of Germany has to be reconsidered. An exchange of plant material between adjacent defined areas of origin should be possible in case of a lack of suitable plant material. This would alleviate the expenditure for the acquisition of seed for tree nurseries and improve the supply of planting stock.
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Received: 4 June 2015 | Revised: 24 August 2015 | Accepted: 17 November 2015 | Published: 22 February 2016 | Available online: 22 February 2016