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  Eur.J.Hortic.Sci. 80 (5) 225-230 | DOI: 10.17660/eJHS.2015/80.5.4
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2015 | European Journal of Horticultural Science | Original article

Maturation changes auxin profile during the process of adventitious rooting in Prunus

G. Osterc and F. Štampar
University of Ljubljana, Biotechnical Faculty, Institute for Fruit Growing, Viticulture and Vegetable Growing, Ljubljana, Slovenia

The changes in indole-3-acetic acid (IAA) and indole-3-acetic acid aspartate (IAA-Asp) levels in leafy cuttings of Prunus subhirtella ‘Autumnalis’ were studied in 2011. Leafy cuttings were severed from mature, semi-mature and juvenile stock plants on 20th of June when a half of them were treated with indole-3-butyric acid (IBA) powder (0.5% IBA + 10% Captan on talcum basis) while the rest remained untreated. Auxins (IAA, IAA-Asp) were measured at the time of severance and after 4 hours, 1 and 2 days. Cuttings from juvenile stock plants rooted significantly better (80%) than cuttings from semi-mature (50%) and mature stock plants (36.7%) and they developed the longest roots (5.19 cm). The IAA-profile in juvenile cuttings reached its maximum (peak) 4 h after severance, whereby the maximum value 4.27 µg g-1 fresh weight (FW) was reached 4 h after severance when the cuttings were pre-treated with IBA. The IAA-profile in semi-mature cuttings reached a peak of 1.48 µg g-1 FW on day 1 after severance but only when the cuttings had been pre-treated with IBA. The IAA-profile in mature cuttings did not reach any significant peak, however the values tended to increase on first day after severance in cuttings without IBA application. The IAA-Asp accumulation ranged between 3.20 and 9.11 µg g-1 FW, which was significantly the strongest in mature cuttings one day after severance without IBA treatment.

Keywords indole-3-acetic acid, indole-3-butyric acid, N-(3-indolacetyl) aspartic acid, juvenility, rhizogenesis

Significance of this study

What is already known on this subject?

  • It was very well known for a long period of time that juvenile material develops roots much easier compared with mature material.
What are the new findings?
  • The IAA-profile in cutting bases differs in cuttings regarding the physiological character of the origin of these cuttings.
What is the expected impact on horticulture?
  • Worse propagation is often not the result of an unsuitable method but the consequence of unsuitable (mature) propagation material.

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Received: 2 June 2014 | Revised: 13 February 2015 | Accepted: 26 March 2015 | Published: 23 October 2015 | Available online: 23 October 2015

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