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  Eur.J.Hortic.Sci. 80 (5) 216-224 | DOI: 10.17660/eJHS.2015/80.5.3
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2015 | European Journal of Horticultural Science | Original article

Characterization and induction kinetics of a putative candidate gene associated with downy mildew resistance in grapevine

C. Liang, L. Liu, C. Zang, K. Zhao and C. Liu
Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, China

Downy mildew in cultivated grapevines (Vitis spp.), caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage due to the repeated application of fungicides. The molecular role of the NBS-LRR family is highly related to plant immune-activity against various pathogens and pests. In this study, the 5′ and 3′ ends of the resistance gene homology fragment, designated as RGA23, were obtained by rapid amplification of cDNA ends PCR (RACE-PCR), and a 2,789 bp full-length cDNA was obtained using the gene-specific primers based on the spliced sequence. The deduced 892 amino acid sequence of this cDNA contains a characteristic NBS-LRR domain of plant resistance genes and a coiled-coil (CC) region. We analyzed the expression of RGA23 under P. viticola infection and abiotic stress at different time points using real-time quantitative polymerase chain reaction (RT-qPCR). The results showed that P. viticola treatment and four tested abiotic stimuli, including SA, MeJA, ABA, and H2O2, triggered significant inductions of RGA23 within 12 d of inoculation. The results indicate that RGA23 may play a critical role in protecting grapevines against P. viticola through a signaling pathway triggered by P. viticola and these molecules.

Keywords coiled-coil region, NBS-LRR, RACE-PCR, RT-qPCR, signaling pathway, Vitis spp.

Significance of this study

What is already known on this subject?

  • To identify grapevine RGAs that are potentially involved in host defense against Plasmopara viticola infection, we previously characterized RGAs of the NBS-LRR family and studied their transcript expression. We then selected the Vitis RGA gene RGA23 for further analysis.
What are the new findings?
  • In this study, the resistance gene homology fragment, designated as RGA23, were obtained by RACE-PCR. We analyzed the expression of RGA23 under P. viticola infection and abiotic stress at different time points using RT-qPCR. The results showed that P. viticola treatment and four tested abiotic stimuli, including SA, MeJA, ABA, and H2O2, triggered significant inductions of RGA23 within 12 d of inoculation.
What is the expected impact on horticulture?
  • Downy mildew, caused by the oomycete Plasmopara viticola, is the most economically important fungal disease of grapes (Vitis spp.). To achieve sustainable grapevine production, the isolation and incorporation of genes into grapevine that confer resistance to downy mildew would be of considerable economic and environmental benefit. The results indicate that RGA23 may play a critical role in protecting grapevines against P. viticola through a signaling pathway triggered by P. viticola and these molecules.

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Received: 28 August 2014 | Revised: 24 November 2014 | Accepted: 10 March 2015 | Published: 23 October 2015 | Available online: 23 October 2015

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