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Journal of the American Pomological Society
(J Am Pom Soc)

American Pomological Society

Volume 60 Number 3 Article 18 Pages: 136-143
Year 2006 Month 7
Title: Simple Sequence Repeat (SSR) Markers Differentiate Turkish Sour Cherry Germplasm
Authors: Y.A. Kacar, M.S. Cetiner, C. Cantini and A.F. Iezzoni
Eighty-one tetraploid cherry selections, including sour cherry (Prunus cerasus L.) and its progenitor species P. fruticosa Pall., were compared using simple sequence repeat (SSR) marker analysis to determine the relationship of germplasm collected from Turkey to those collected from Russia and northern and eastern regions of Europe. SSR fragments were produced with all five primer pair – cherry selection combinations. Cherry selections exhibited high levels of polymorphism with 5 to 20 different putative alleles amplified per primer pair. The primer pair PMS 49, isolated from sweet cherry, showed a high level of polymorphism with 20 putative alleles identified. However, SSR analysis was not able to differentiate among some selections. Overall, Turkish selections tended to group together and separated from other cherry selections. The Turkish germplasm did not exhibit many of the putative SSR alleles identified in the broader germplasm pool; however, it exhibited many novel alleles. This study demonstrated the importance of including germplasm from Turkey, an important ancestral region for sour cherry, when building a germplasm collection. It also demonstrated the utility of SSR markers in identifying genetic variation “gaps” within a germplasm collection.

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