Volume 57 Number 3 Article 18
Year 2003 Month 7
Title: Identification and Genetic Relationship of Persian Walnut Genotypes Using Isozyme Markers
Authors: A. Ninot and N. Aleta
Abstract:
Horizontal starch gel electrophoresis was used to identify 108 walnut genotypes and to assess their
genetic relationship.
The DIA, 6-PGD, PGM, SDH, MDH and MPI enzyme systems were encoded for
12 loci, 8 of which were polymorphic.
With just three systems (DIA, 6-PGD, PGM), nearly 70% of the
studied genotypes were identified.
Four systems (DIA, 6-PGD, PGM, SDH) allowed for more than 80%
to be identified and all 6 systems studied made it possible to distinguish over 90%. Eight loci were found
to be more than sufficient to identify 91 of the 108 genotypes studied.
The Nei and Li index suggests that walnut germplasm is very closely related, with an average value
among all genotypes of 0.775. Cultivars from Romania ('Oprean*, 'Maria Mamma' and 'Sibisel-39')
showed markedly lower similarity indices than the other cultivars.
The UPGMA dendrogram based on the similarity matrix separated the walnut genotypes included in
this study into two clear groups.
The first group consisted of two sub-clusters, one of which mainly comprised genotypes from Spanish, French, Chilean and other European cultivars.
The other sub-cluster
consisted primarily of Californian cultivars closely related to the 'Payne' cultivar.
The second main
group, though much smaller in size, mainly comprised genotypes from Iran and Romania.
Isozyme
markers have proven to be useful tools for identifying and fingerprinting walnut cultivars.
Full text download: APS subscribers
ISHS members & pay-per-view
(PDF 1041985 bytes)
Translate:
APS membership administration
ISHS membership administration
