Volume 50 Number 2 Article 18
Year 1996 Month 4
Title: Technique Development Towards Production of an In Vitro Graft Chimera in Rubus
Authors: H.J. Chen, M.M. Mubarack, S.K. Naess, E. Stover, and H.J. Swartz
Abstract:
In vitro grafting was attempted to produce
periclinal chimeras in Rubus. The effect of
plant growth regulators on the preparation of
graf table material, in vitro grafting and regeneration of shoots from graft union tissue was
investigated on two thornless and two thorny
blackberries.
Grafted plants grown on medium
containing no plant growth regulators, 1 µM
abscisic acid or 10 µM paclobutrazol produced
79 to 84% successful grafts compared to 20 to
50% successful grafts on medium containing
Ethrel or indolebutryic acid.
Percentage shoot
formation from excised graft unions ranged
between 19 and 40%, only 5 µM indole butyric
acid significantly reduced regeneration from
the more organogenic treatments.
Regenerant
shoots were primarily from the scion in ethrel,
no plant growth regulator and paclobutrazol
treated graft unions.
Other treatments resulted
in equal numbers of shoots with scion and
rootstock phenotypes.
Although over 200 regenerants
were obtained from both sides of the
graft union, no thornless/thorny chimeras were
recovered from the subsequent use of this
technique.
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