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Journal of the American Pomological Society

American Pomological Society

Volume 50 Number 2 Article 15
Year 1996 Month 4
Title: Liquid Medium Overlays Enhance Growth and Multiplication of In Vitro Grapes and Apples
Authors: M. Ali-Ahmad, H.G. Hughes, F. Safadi and I. Dami
Abstract:
Liquid medium overlays of established cultures were compared to transfers to fresh medium. The number of micropropagated grape (Vitis sp.) shoots increased 67%, shoot length 73%, and fresh weight 125% with liquid medium overlays as compared to transfer to fresh me dium. Callus growth at the base of shoots was 64$ less. In apples (Malus pumila), the number of micropropagated shoots increased 35%, shoot length 69%, and fresh weight 67% with liquid medium overlays. Callus fresh weight decreased by 52% when compared to cultures transferred to fresh solid media.

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