Volume 50 Number 2 Article 13
Year 1996 Month 4
Title: New Tetraploid Breeding Parents for Triploid Seedless Citrus Cultivar Development
Authors: F.A.A. Mourao Fo, F.G. Gmitter, Jr. and J.W. Grosser
Abstract:
Protoplast culture following polyethylene
glycol (PEG)-induced fusion resulted in the
regeneration of somatic hybrid plants from
'Succari' sweet orange (C. sinensis L. Osbeck)
with 'Dancy' tangerine (C. reticulata Blanco),
'Minneola' tangelo (C. paradisi Macf. × C.
reticulata Blanco), 'Murcott' tangor (purported
C. reticulata × C. sinensis), 'Page' tangelo ['Minneola'
tangelo × 'Clementine' mandarin (C. reticulata)], and 'Ponkan' mandarin (C. reticulata).
'Succari' protoplasts were isolated from ovulederived
embryogenic cell suspension cultures,
and protoplasts of the mandarin-type parents
were isolated from seedling leaves.
Somatic
hybrid plants were identified on the basis of
leaf morphology, root tip chromosome number,
and isozyme and RAPD analyses.
These tetraploid plants may have direct cultivar potential;
however, their greatest value will be as pollen
parents to be crossed with selected monoembryonic
diploids to produce seedless triploids.
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