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Journal of the American Pomological Society

American Pomological Society

Volume 47 Number 4 Article 2
Year 1993 Month 10
Title: Changes in Levels and Isozymes of Peroxidase in Wounded Peach Bark
Authors: M.M. Goodin, A.R. Biggs and A.M. Castle
Abstract:
Bark on one-yr-old branches from six peach cultivars was mechanically wounded then exam ined over time for changes in peroxidase iso zymes, total peroxidase activity, and total pro tein. Four peroxidase isozymes were identified and changes in total activity were associated mostly with one isozyme. Changes in peroxidase activity in response to wounding were observed with activity increasing to the highest levels at 4 and 7 days post-wounding. At 10 and 14 days post-wounding, peroxidase levels had returned to levels similar to the control. An unidentified would response protein was detected in these experiments. The timing of the appearance of this protein was correlated with the known relative susceptibility of the cultivars to the peach canker pathogens, Leucostoma persoonii and L. cincta. Comparisons of the peroxidase isozyme profiles and total peroxidase activity among cultivars provided no indication that peroxidase profiles could be used as a marker for resistance to the peach canker pathogens.

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