ISHS Acta Horticulturae 850: III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics
STUDIES IN RELATION TO MOLECULAR VARIABILITY IN SAFFRON |
| Authors: | S. Imran, F.A. Nehvi, S.A. Wani, G. Zaffar, M.A. Khan |
| Keywords: | Crocus sativus L., diversity, RAPD |
| Abstract:
Molecular characterization of ten elite saffron genotypes based on 21 Random Amplified Polymorphic DNA (RAPD) markers revealed a considerable amount of genetic diversity among tested genotypes. Similarity index based on Jaccards coefficient ranged from 0.375 to 0.834 and the maximum similarity coefficient (0.834) was observed between the genotype SMD-45 and SMD-79. The dendrogram based on molecular data divided the tested genotypes in two clusters. However, genotypes viz., SD-3, SMD-45, SMD-79 and SMD-68 formed the cluster I and genotypes viz., SMD-11, SMD-52, SMD-81, SMD-211, SMD-224 and control formed cluster II at similarity coefficient of 44%,which showed a high level of genetic diversity between two clusters containing different genotypes. A different level of variability was observed among different genotypes within each cluster also. The primer pair i.e., SA-D+SA-S was found to be the best primer pair which showed maximum number of scorable and polymorphic bands. | |
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