ISHS Acta Horticulturae 850: III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics
DETECTION OF ERYTHROSINE IN SAFFRON |
| Authors: | S.A. Ordoudi, K. Tsioga, M.Z. Tsimidou |
| Keywords: | Crocus sativus L., adulteration, synthetic colorants, fluorescence, photodegradation |
| Abstract:
Erythrosine (E127, CI 45430) is not easily detected in adulterated saffron under the TLC and HPLC conditions proposed by ISO (3632-2, 2003) specifications for identification of artificial colorants in this precious plant material. In this study fluorescence is introduced for the first time as a means of detection after sample clean-up prior chromatographic separation according to aforementioned specifications at this stage of analysis. The mimimum required performance limit (MRPL) was found lower than that obtained using UV-Vis spectrophotometry by two-orders of magnitude. After chromatographic separation of erythrosine fluorescence detection at the same addition levels was less successful. | |
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