ISHS Acta Horticulturae 850: III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics
MANUAL PROCEDURE TO ASSESS PRO-/ANTIOXIDANT IMBALANCE IN HUMAN PLASMA BASED ON THE CROCIN BLEACHING ASSAY (CBA) |
| Authors: | S.A. Ordoudi, N. Nenadis, M.Z. Tsimidou, M. Sarigianni, K. Paletas |
| Keywords: | Crocin Bleaching Assay, ORAC, oxidative stress, serum |
| Abstract:
Crocin Bleaching Assay (CBA) was applied to serum samples randomly collected from 22 obese donors, in order to investigate whether it could detect patients suffering from metabolic syndrome (MS), which is associated with oxidative stress. The same samples were also tested using the ORAC method, having analytical similarities with CBA (pH value, radical initiator and temperature) and commonly applied to biological fluids. Statistical analysis indicated that CBA was more promising in predicting the MS in obese subjects. The current study contributes to the commercial exploitation of saffron in the field of analytical chemistry. | |
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