ISHS Acta Horticulturae 850: III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics
THE FAMILY OF MADS-BOX TRANSCRIPTION FACTORS CONTROLLING FLOWER FORMATION IN CROCUS SATIVUS L. |
| Authors: | A.S. Tsaftaris, K. Pasentsis, A. Kalivas, A. Argiriou, A.N. Polidoros |
| Keywords: | Crocus sativus L., flowering, MADS box, Rolling Circle Amplification |
| Abstract:
Flowering and flower formation is a major developmental switch in plants where different genes are expressed in a coordinate manner for reproductive success. In order to uncover and understand the molecular mechanisms controlling flowering and flower formation in cultivated Crocus sativus L. and to characterize a number of crocus flower mutants, we have cloned and characterized different cDNA and genomic sequences encoding MADS-box transcription factor proteins involved in flowering and flower formation using a combination of different methods including conventional 5’ and 3’ RACE, as well as newly developed methods to isolate full cDNAs and genomic sequences based on Rolling Cycle Amplification (RCA). | |
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