|Authors: ||N.O. Anderson, A. Younis, E. Optiz|
|Keywords: ||interspecific hybridization, hybrid breakdown, VER1, VER2, self incompatibility, self compatibility, ISSRs|
Recent discovery of seed-propagated Lilium × formolongi hybrids that flower continuously in <1 yr without vernalization (200-240d after sowing due to ≥1 dominant VER1, VER2 alleles) with frost-tolerance, day neutrality, and winter hardiness is an unprecedented combination in Lilium. Our objective to obtain colored L. × formolongi was to use Class I lilies that initiate flower buds prior to a cold treatment as parents, e.g. Lilium martagon. Reciprocal interspecific crosses between fertile parents (1-L. × formolongi, 7-colored L. martagon) were made in both directions to generate segregating hybrid (F1) and inbred (F2) progenies.
A total of 8,826 F1 seeds or embryos were generated.
Embryo rescue was employed when L. martagon was the female, although viable embryos never germinated after ~1 yr in culture.
Mean germination ranged from 0-1.02% for in situ ripened seed.
Hybridity for one successful cross (07L-14; L. × formolongi [00L-111-343×51-202-1] × L. martagon ‘Cadense’) was analyzed using morphological (flowering, leaf and internode number, leaf length:width ratios, compatibility, no. flower stalks and flowers, rosetting) and molecular markers (ISSR primers). Twenty-two 07L-14 genotypes were genetically similar to the female parent whereas 86 aligned closely with the male (‘Cadense’). The number of shoots/plant was the only quantitative trait co-segregating with ISSRs.
Flowering traits were intermediate to, but significantly different than, either parent.
A majority of the plant height and inflorescence lengths were significantly greater than the parents.
Two F1s had slight flower coloration in the petals and tepals; one hybrid had an open-faced rather than trumpet flower (similar to an Oriental type).
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