ISHS Acta Horticulturae 789: XV Meeting of the EUCARPIA Tomato Working Group
DNA-MICROARRAY DETECTION OF MOLECULAR MARKERS FOR IMPROVING COLOR AND NUTRITIONAL QUALITY IN TOMATO |
| Authors: | A. Darrigues, W. Yang, D.M. Francis |
| Keywords: | QTL, yellow shoulder disorder, carotenoid |
| Abstract:
Color is among the most important attributes of tomato for processing into whole and diced products. Both color and color uniformity are greatly affected by Yellow Shoulder Disorder (YSD), a ripening disorder that results in discoloration of the proximal end tissues of the fruit. We show that lycopene and  -carotene concentrations are reduced by 18% and 22%, respectively, in fruits affected by YSD. Variance partitioning suggests that YSD incidence and severity is affected by both genetics and environment.
In order to elucidate the genetic basis of YSD, we are developing single nucleotide polymorphisms (SNPs) as molecular markers for application in three inbred backcross populations derived from either Solanum lycopersicum × S. lycopersicum or S. lycopersicum × S. pimpinellifolium crosses.
SNP discovery for application in these populations is based on both analyses of large public EST databases and on hybridization to a custom oligonucleotide array.
The array was hybridized with target cDNA from S. lycopersicum (Ohio 7814) and S. pimpinellifolium (LA1589). We developed algorithms to detect outliers and identified 1,296 potential SNPs.
These putative SNPs are being verified by sequencing, screened for utility as markers on a collection of 99 S. lycopersicum lines and wild relatives and applied to the genetic dissection of YSD. Implementing SNP-based marker technology has the potential to dramatically alter our approach to genetic characterization.
Results and interpretation from this study will help bridge the gap between the goals of genetic and crop improvement research by facilitating the use of population structures that favor simultaneous genetic analysis and crop improvement.
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