ISHS Acta Horticulturae 787: International Workshop on Tropical and Subtropical Fruits
DEVELOPMENT OF ADVANCED TECHNOLOGIES FOR GERMPLASM CONSERVATION OF TROPICAL FRUIT SPECIES - CITRUS AND NEPHELIUM SPP. |
| Authors: | S. Somsri, J. Chartisathian, S. Suntaranond |
| Keywords: | cryopreservation, embryogenic callus, desiccation, encapsulation, dehydration, vitrification |
| Abstract:
Polyembryonic citrus seeds cryopreservation method using desiccation and embryonic callus cryopreservation method using new encapsulation dehydration were studied. Also, monoembryonic citrus cryopreservation method using new encapsulation dehydration and shoot-tip grafting were investigated. In Rambutan, it was found that the percentage of survival dramatically decreased when the moisture content of axes droped below 50%. Longer pre-growth and pre-treatment reduced viability percentage prior to expose to LN. No survival was obtained after cryopreservation in LN either using encapsulation or vitrification. | |
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