ISHS Acta Horticulturae 781: XX International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops - Fruit Tree Diseases
FIRST RECORD OF PEACH LATENT MOSAIC VIROID IN CROATIA |
| Authors: | D. Škorić, M. Al Rwahnih, A. Myrta |
| Keywords: | PLMVd, nectarine, peach, tissue-printing, RT-PCR |
| Abstract:
Throughout 2003 and 2004, 168 Prunus trees were analyzed by tissue-printing molecular hybridization for the presence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). This survey was carried out in different Croatian continental and coastal (Mediterranean) stone fruit growing regions. The sampling encompassed various cultivated stone fruit species (peach, nectarine, apricot, sweet and sour cherry), wild Prunus (P. myrabolana, P. mahaleb, P. padus, P. spinosa) as well as urban ornamentals (P. juliana, P. laurocerasus, P. serrulata, P. subhirtela and P. virginiana). The trees were located in commercial orchards, stone fruit collections, family gardens, or they were sampled as individual urban trees. The collection of ornamental Prunus from the Botanical Garden of the Department of Biology in Zagreb was also included in this survey. PLMVd was detected only in nectarine and peach trees with the incidence of 94% and 42%, respectively. Infected nectarines, originating from Italy, were grown in both climatic regions. PLMVd infected peaches originated both from imported and native cultivars. The PLMVd-positive samples obtained by tissue printing hybridization were confirmed by RT-PCR. None of the randomly tested wild Prunus or the ornamental trees had viroid-positive results. This is the first record of PLMVd in Croatia. This preliminary survey can help in developing comprehensive testing schemes and strategies for stone fruit tree viroid control. | |
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