ISHS Acta Horticulturae 765: XXVII International Horticultural Congress - IHC2006: International Symposium on Plants as Food and Medicine: The Utilization and Development of Horticultural Plants for Human Health
DETERMINATION OF THE INHERITANCE OF PUNGENCY IN CAPSICUM BY MOLECULAR TECHNIQUES |
| Authors: | S. Techawongstien, S. Khemnak, P. Theerakulpisut |
| Keywords: | chilli breeding program, capsaicinoid, broad-sense heritability, DNA fingerprint, SCAR-primers |
| Abstract:
In order to determine the inheritance of pungency in chilli pepper more accurately and precisely, molecular techniques were used as tools together with conventional techniques in a chilli breeding program. In this study, the highly pungent variety “Yodson” (YS), which was the local commercial variety in Thailand, was selected and crossed with the low pungency variety from The Peoples Republic of China, “YuYi” (YY). The capsaicinoid content, broad-sense heritability (hb2), and DNA fingerprint by bulked-segregant analysis with UBC-20 SCAR primer were compared among F1-hybrid and F2 populations and their parents. The capsaicinoid contents of the F1 and F2 generations were high, similar to the high pungency parent (YS). Meanwhile, the hb2 in capsaicinoid content of the crosses was also quite high (~0.69). The SCAR primer for UBC-20 could amplify a common fragment of 1700 bp in both low and high pungency chilli plants of all generations. However, the fragment of 1700 bp was observed after digestion with HindIII only in the high pungency plants compared to the two fragments of 1300 and 400 bp in both high and low pungency plants. | |
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