ISHS Acta Horticulturae 763: XXVII International Horticultural Congress - IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants
EVALUATION OF INCOMPATIBILITY IN FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) CULTIVARS USING PCR |
| Authors: | M. Koushesh-Saba, K. Arzani, M. Jalali-Javaran |
| Abstract:
Pear fruit set is affected by several genetic and environmental factors including incompatibility. Self-incompatibility (SI) is controlled in Rosaceae by a homomorphic, gametophytic, monofactorial, multiallelic incompatibility system and pollen rejection occurs in the style. The incompatibility locus controls self-incompatibility as well as intra-specific cross-incompatibility. The present research was carried out during the 2004 growing season at Tarbiat Modares University (TMU) Iran, in order to evaluate incompatibility in five European pear (Pyrus communis L.) cultivars. Polymerase chain reaction (PCR) with specific primers was applied to investigate SI genes in pear cultivars including 'Shah-Miveh', 'Spadona', 'Sar-drood', 'Mohammad-Ali' and 'Bartlet'. DNA was extracted according to the modified CTAB method and the SI gene was amplified using specific primers. The results indicated that primers could amplify the SI gene and that a S-locus existed in these cultivars. The first pair of primers showed that there was one pair of S1, S3-S7 alleles in 'Shah-Miveh' and 'Mohammad-Ali'. A similar band pattern with S2 allele was observed in ‘Sar-drood’ and ‘Bartlet’ cultivars. In addition, in 'Spadona', 'Sar-drood' and 'Bartlet' a 1400 base pair band was amplified suggesting the existence of a possible new allele. Similar results were observed using other primers. In conclusion, we suggest using a suitable combination of cultivars with different S alleles for newly established pear orchards and breeding programs. | |
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