ISHS Acta Horticulturae 763: XXVII International Horticultural Congress - IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants
MAPPING QUANTITATIVE TRAIT LOCI EFFECTING CUCUMBER CARPOPODIUM LENGTH USING SIMPLE SEQUENCE REPEAT MARKERS |
| Authors: | Guiling Wang, Zhiwei Qin, Xiuyan Zhou, Zhiyun Zhao |
| Keywords: | Cucumis sativus, QTL |
| Abstract:
Use of molecular markers for selection of important agronomic traits can enhance breeding efficiency. An F2 population from a cross between 129, a cucumber line with long carpopodium, and Z3, a cultivar with short carpopodium, was used to identify quantitative trait loci (QTLs) associated with cucumber carpopodium length. DNA was extracted from F2 plants and amplified using a simple sequence repeat (SSR) marker. A total of five polymorphic SSR markers were used for QTL detection. Based on the data recorded, those five markers were used to construct the genetic linkage map using mapmaker 3.0b. QTLs were detected with Windows QTL Cartographer V2.0. Four markers CSWGATT01B, CSWGATT01C, CSCT33 and CSWGATT01A, from one linkage group, were significantly associated with carpopodium length. Qchl1 was linked with SSR maker CSWGATT01B and CSWGATT01C by 3.4 cM and 18.0 cM, respectively. This explained 18.49% of phenotypic variance. | |
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