ISHS Acta Horticulturae 760: XXVII International Horticultural Congress - IHC2006: II International Symposium on Plant Genetic Resources of Horticultural Crops
CRYOPRESERVATION OF SOME MEDITERRANEAN SMALL FRUIT PLANTS |
| Authors: | C. Damiano, M.D. Arias Padró, A. Frattarelli |
| Keywords: | genetic resources, hawthorn, preservation, service tree, strawberry tree |
| Abstract:
Minor fruit species are increasingly utilized as dietary complements, in the pharmaceutical industry, in reforestation programs and for ornamental purposes. However, due to the fact that cultivation of such species is limited to marginal areas, there is an increasing risk of losing these genetic resources; therefore it is necessary to develop new approaches to preserve their biodiversity. Research on the preservation of fruit crops was initiated some years ago and today the interest for its application is increasing world wide. Two alternative ex situ conservation strategies are available to augment field planting: short-term storage of in vitro cultures and long-term storage of shoot apices in cryopreservation in liquid nitrogen. Previous studies have described other problems related to in vitro preservation, such as oxidization and/or necrotizetion of explants. The objective of this research was to develop a protocol for germplasm conservation of in vitro apices of hawthorn (Crataegus azarolus), service tree (Sorbus domestica) and strawberry tree (Arbutus unedo). Results showed that the encapsulation-dehydration technique can be used successfully with regrowth percentages after cryo-preservation of 25% in hawthorn, 25% in the service tree and 45% in the strawberry tree. | |
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