ISHS Acta Horticulturae 726: IV International Symposium on Pistachios and Almonds
A STUDY ON GENETIC VARIATION AMONG SOME WILD ALMOND SPECIES USING RAPD MARKERS |
| Authors: | S. Kadkhodaei, S.R.T. Aghdaei, V. Grigorian, M. Moghadam, S.M.M. Hashemi |
| Keywords: | genetic variation, Iran, RAPDs, wild almonds |
| Abstract:
The genetic variation of 12 natural populations of wild almond (Amygdalus spp. = Prunus amygdalus Batsch.) growing in the province of Isfahan (central region of Iran) as well as some cultivated almonds was assessed using RAPDs. Genomic DNA was extracted from young-leaf tissues. Nine out of the selected 10-mer primers amplified polymorphic DNA bands in all individuals. The resulting bands sizes varied from 200 to 3229 bp. Jaccard’s similarity coefficients and UPGMA were used to produce a cluster diagram. Based on this method populations were divided into four groups, each belonging to one of the four almond species included A. scoparia, A. haussknechtii, A. lycioides and A. elaegnifolia. Overall, a relatively high degree of diversity was detected both within and between populations. According to the mean intraspecific similarity coefficients, the genetic variation in A. haussknechtii was found to be greater than in the other species. With interspecific variation, the greatest genetic distance was between A. lycioides and A. haussknechtii. So regarding the relatively high and more exact demonstrated diversity among these plants, it can be utilized in breeding of valuable and important traits and thus it can provide the possibility for consideration of useful genes in new individuals. | |
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